LEDGF binds to heat shock and stress-related element to activate the expression of stress-related genes

Dhirendra P. Singh, Nigar Fatma, Akira Kimura, Leo T. Chylack, Toshimichi Shinohara

Research output: Contribution to journalArticlepeer-review

117 Scopus citations

Abstract

We have investigated the mechanism by which LEDGF protects cells against environmental stress. Our earlier report showed that a low level of LEDGF was present in the nucleus of most cell types and significant elevation of LEDGF level was induced by heat and oxidative stress. The cells overexpressing LEDGF-activated expression of heat shock proteins and enhanced survival of many cell types. Here we show that LEDGF binds to heat shock element (HSE) and stress-related regulatory element (STRE) to activate the expression of stress-related genes (Hsp27 and αB-crystallin). Apparently, HSE and STRE are present in promoters of many stress-related genes. Elevation of many stress-related proteins (STRPs) induced by LEDGF may protect cells against environmental stress. In yeast, it has been demonstrated that a single stress can activate the expression of multiple STRPs. This is known as "cross-protection," and now similar mechanism has been found in mammalian cells and LEDGF plays a vital role in it.

Original languageEnglish (US)
Pages (from-to)943-955
Number of pages13
JournalBiochemical and Biophysical Research Communications
Volume283
Issue number4
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Consensus core elements
  • Cross-protection
  • Hsp27
  • LEDGF
  • Stress-related promoter
  • αB-crystallin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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