Abstract
We have investigated the mechanism by which LEDGF protects cells against environmental stress. Our earlier report showed that a low level of LEDGF was present in the nucleus of most cell types and significant elevation of LEDGF level was induced by heat and oxidative stress. The cells overexpressing LEDGF-activated expression of heat shock proteins and enhanced survival of many cell types. Here we show that LEDGF binds to heat shock element (HSE) and stress-related regulatory element (STRE) to activate the expression of stress-related genes (Hsp27 and αB-crystallin). Apparently, HSE and STRE are present in promoters of many stress-related genes. Elevation of many stress-related proteins (STRPs) induced by LEDGF may protect cells against environmental stress. In yeast, it has been demonstrated that a single stress can activate the expression of multiple STRPs. This is known as "cross-protection," and now similar mechanism has been found in mammalian cells and LEDGF plays a vital role in it.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 943-955 |
| Number of pages | 13 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 283 |
| Issue number | 4 |
| DOIs | |
| State | Published - 2001 |
| Externally published | Yes |
Keywords
- Consensus core elements
- Cross-protection
- Hsp27
- LEDGF
- Stress-related promoter
- αB-crystallin
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology