TY - JOUR
T1 - Lens epithelium-derived growth factor
T2 - Effects on growth and survival of lens epithelial cells, keratinocytes, and fibroblasts
AU - Singh, Dhirendra P.
AU - Ohguro, Nobuyuki
AU - Kikuchi, Takanobu
AU - Sueno, Toshiharu
AU - Reddy, Venkat N.
AU - Yuge, Kenshi
AU - Chylack, Leo T.
AU - Shinohara, Toshimichi
N1 - Funding Information:
We are grateful to Dr. Joseph Horwitz (UCLA School of Medicine) for providing human capsulotomy specimens and to Dr. George Thurston for his critical reading of the manuscript. This study was supported in part by Shojin Research Associates (Studio City, CA), the Brigham Surgical Group Foundation, the Massachusetts Lions Eye Research Fund, Inc., the following NIH-sponsored RO1 project grants (EY-00484, EY-05230, EY-12015), and the Japan Eye Bank Associate Fund.
PY - 2000/1/7
Y1 - 2000/1/7
N2 - We isolated a clone encoding a protein from a human lens epithelial cell (LEC) cDNA library with antibody (Ab) from a cataract patient and named it ″lens epithelium-derived growth factor″ (LEDGF). LEDGF is found to be identical to p75, a coactivator of both transcription (1) and pre-mRNA splicing (2). In serum-free medium LEDGF stimulated growth of LECs, cos7 cells, skin fibroblasts, and keratinocytes, and prolonged cell survival. Without LEDGF, the aforementioned cells did not survive. Also in serum-free medium, Ab to LEDGF neutralizing LEDGF blocked cell growth and caused cell death. Thus, LEDGF, a regulatory factor, may play an important role for growth and survival of a wide range of cell types. (C) 2000 Academic Press.
AB - We isolated a clone encoding a protein from a human lens epithelial cell (LEC) cDNA library with antibody (Ab) from a cataract patient and named it ″lens epithelium-derived growth factor″ (LEDGF). LEDGF is found to be identical to p75, a coactivator of both transcription (1) and pre-mRNA splicing (2). In serum-free medium LEDGF stimulated growth of LECs, cos7 cells, skin fibroblasts, and keratinocytes, and prolonged cell survival. Without LEDGF, the aforementioned cells did not survive. Also in serum-free medium, Ab to LEDGF neutralizing LEDGF blocked cell growth and caused cell death. Thus, LEDGF, a regulatory factor, may play an important role for growth and survival of a wide range of cell types. (C) 2000 Academic Press.
KW - Age-related cataract
KW - Autoantibody
KW - Autocrine
KW - Lens epithelium-derived growth factor (LEDGF)
KW - Survival factor
KW - Trafficking
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U2 - 10.1006/bbrc.1999.1979
DO - 10.1006/bbrc.1999.1979
M3 - Article
C2 - 10623627
AN - SCOPUS:0034614581
SN - 0006-291X
VL - 267
SP - 373
EP - 381
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -