Lens epithelium-derived growth factor: Effects on growth and survival of lens epithelial cells, keratinocytes, and fibroblasts

Dhirendra P. Singh, Nobuyuki Ohguro, Takanobu Kikuchi, Toshiharu Sueno, Venkat N. Reddy, Kenshi Yuge, Leo T. Chylack, Toshimichi Shinohara

Research output: Contribution to journalArticlepeer-review

130 Scopus citations

Abstract

We isolated a clone encoding a protein from a human lens epithelial cell (LEC) cDNA library with antibody (Ab) from a cataract patient and named it ″lens epithelium-derived growth factor″ (LEDGF). LEDGF is found to be identical to p75, a coactivator of both transcription (1) and pre-mRNA splicing (2). In serum-free medium LEDGF stimulated growth of LECs, cos7 cells, skin fibroblasts, and keratinocytes, and prolonged cell survival. Without LEDGF, the aforementioned cells did not survive. Also in serum-free medium, Ab to LEDGF neutralizing LEDGF blocked cell growth and caused cell death. Thus, LEDGF, a regulatory factor, may play an important role for growth and survival of a wide range of cell types. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)373-381
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume267
Issue number1
DOIs
StatePublished - Jan 7 2000
Externally publishedYes

Keywords

  • Age-related cataract
  • Autoantibody
  • Autocrine
  • Lens epithelium-derived growth factor (LEDGF)
  • Survival factor
  • Trafficking

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Lens epithelium-derived growth factor: Effects on growth and survival of lens epithelial cells, keratinocytes, and fibroblasts'. Together they form a unique fingerprint.

Cite this