Lens epithelium-derived growth factor: Increased resistance to thermal and oxidative stresses

Dhirendra P. Singh, Nobuyuki Ohguro, Leo T. Chylack, Toshimichi Shinohara

Research output: Contribution to journalArticlepeer-review

105 Scopus citations

Abstract

PURPOSE. To investigate the role of lens epithelium- derived growth factor (LEDGF) in lens epithelial cells subjected to heat or oxidative stress and to determine the localization of LEDGF in lens epithelial cells at different temperatures. METHODS. Mouse lens epithelial cells overexpressing a fusion protein between a green fluorescent protein (GFP) and LEDGF (GFP- LEDGF) were cultured for up to 7 days at various temperatures or for 24 hours in the presence of hydrogen peroxide. Translocation of GFP-LEDGF in the lens epithelial cells was monitored with a fluorescence microscope. Cell survival was determined with the trypan blue dye exclusion test. Expression of heat shock proteins (Hsps) was studied with protein blot analyses using antibody probes. RESULTS. LEDGF was found in the cytosol of lens epithelial cells at 4°C, 15°C, and 28°C, and in the nucleus and nucleolus at 37°C. At 41 °C, it was apparent at higher levels in the cytosol, nucleus, and nucleolus. Lens epithelial cells overexpressing LEDGF manifested resistance to thermal and oxidative stress. Levels of Hsp 27, and αB-crystallin were elevated in these cells. Depriving lens epithelial cells of LEDGF with anti-LEDGF antibodies reduced nuclear localization of LEDGF and induced cell death. CONCLUSIONS. The overexpression of LEDGF in lens epithelial cells conferred resistance to thermal and oxidative stress. The mechanism of this resistance appears to involve the increased expression of Hsp 27 and αB-crystallin.

Original languageEnglish (US)
Pages (from-to)1444-1451
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume40
Issue number7
StatePublished - 1999
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Lens epithelium-derived growth factor: Increased resistance to thermal and oxidative stresses'. Together they form a unique fingerprint.

Cite this