Lens epithelium-derived growth factor (LEDGF/p75) and p52 are derived from a single gene by alternative splicing

Dhirendra P. Singh, Akira Kimura, Leo T. Chylack, Toshimichi Shinohara

Research output: Contribution to journalArticlepeer-review

83 Scopus citations

Abstract

A human gene that encodes lens epithelium-derived growth factor (LEDGF) was isolated, and the DNA sequence and the exon/intron organization was determined. The gene contains at least 15 exons and 14 introns and encodes LEDGF mRNA and p52 mRNA. Exons 1-15 encode LEDGF mRNA, and exons 1-9, and a part of the ninth intron encode a splice variant (p52). Sequences of the exon/intron junctions of the gene have the highly conserved GT/AG rule. Most intron/exon junctions correspond to junctions of individual protein motifs. Almost equal amounts of LEDGF and p52 are expressed in lens epithelial cells in culture. The LEDGF gene is assigned to chromosome 9p22.2, which is adjacent to the major cell malignancy locus.

Original languageEnglish (US)
Pages (from-to)265-273
Number of pages9
JournalGene
Volume242
Issue number1-2
DOIs
StatePublished - Jan 25 2000
Externally publishedYes

Keywords

  • Alternative splice
  • Chromosome 9p22.2
  • Exon/intron
  • Gene
  • LEDGF/p75 and p52

ASJC Scopus subject areas

  • Genetics

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