Leptospira genomes are modified at 5'-GTAC

D. Ralph, Q. Que, J. L. Van Etten, M. McClelland

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Genomic DNAs of 14 strains from seven species of the spirochete Leptospira were resistant to cleavage by the restriction endonuclease RsaI (5'-GTAC). A modified base comigrating with m4C was detected by chromatography. Genomic DNAs from other spirochetes, Borrelia group VS461, and Serpulina strains were not resistant to RsaI digestion. Modification at 5'-GTAm4C may occur in most or all strains of all species of Leptospira but not in all genera of spirochetes. Genus-wide DNA modification has rarely been observed in bacteria.

Original languageEnglish (US)
Pages (from-to)3913-3915
Number of pages3
JournalJournal of bacteriology
Issue number12
StatePublished - 1993
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology


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