Leukotriene D4 potentiates fibronectin-induced migration of human lung fibroblasts

Jun Kato, Tadashi Kohyama, Hitoshi Okazaki, Masashi Desaki, Takahide Nagase, Stephen I. Rennard, Hajime Takizawa

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Fibroblasts play an important role in the repair and remodeling processes following injury. Leukotriene D4 (LTD4) is a potent mediator in inflammatory processes, but the direct effect of cysteinyl leukotrienes on fibroblast migration remains unelucidated. In this study, the effect of the LTD4 on normal human lung fibroblasts (NHLF) chemotaxis induced by human plasma fibronectin (HFn) was investigated using the modified Boyden's chamber technique. LTD4 potentiated NHLF chemotaxis to HFn in concentration-dependent manner. A specific cysteinyl leukotriene receptor type 1 antagonist, pranlukast inhibited this effect, indicating that LTD 4 affected cell migration via its specific receptor. The potentiating effect of LTD4 on fibroblast chemotaxis was completely abolished by pertussis toxin (PTX), suggesting that LTD4-induced effect was dependent on PTX-sensitive Gi/o signaling. These findings suggest that LTD 4 has a potential to augment fibroblast chemotaxis, and to contribute to regulation of the wound healing and following remodeling in fibrotic processes of the lung.

Original languageEnglish (US)
Pages (from-to)177-181
Number of pages5
JournalClinical Immunology
Volume117
Issue number2
DOIs
StatePublished - Nov 2005

Keywords

  • Airway remodeling
  • Cysteinyl leukotrienes
  • Fibroblast
  • Fibrosis
  • Migration

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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