TY - JOUR
T1 - Lifestyle and horizontal gene transfer- mediated evolution of Mucispirillum schaedleri, a core member of the murine gut microbiota
AU - Loy, Alexander
AU - Pfann, Carina
AU - Steinberger, Michaela
AU - Hanson, Buck
AU - Herp, Simone
AU - Brugiroux, Sandrine
AU - Neto, João Carlos Gomes
AU - Boekschoten, Mark V.
AU - Schwab, Clarissa
AU - Urich, Tim
AU - Ramer-Tait, Amanda E.
AU - Rattei, Thomas
AU - Stecher, Bärbel
AU - Berry, David
N1 - Publisher Copyright:
Copyright © 2017 Loy et al.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Mucispirillum schaedleri is an abundant inhabitant of the intestinal mucus layer of rodents and other animals and has been suggested to be a pathobiont, a commensal that plays a role in disease. In order to gain insights into its lifestyle, we analyzed the genome and transcriptome of M. schaedleri ASF 457 and performed physiological experiments to test traits predicted by its genome. Although described as a mucus inhabitant, M. schaedleri has limited capacity for degrading host-derived mucosal glycans and other complex polysaccharides. Additionally, M. schaedleri reduces nitrate and expresses systems for scavenging oxygen and reactive oxygen species in vivo, which may account for its localization close to the mucosal tissue and expansion during inflammation. Also of note, M. schaedleri harbors a type VI secretion system and putative effector proteins and can modify gene expression in mucosal tissue, suggesting intimate interactions with its host and a possible role in inflammation. The M. schaedleri genome has been shaped by extensive horizontal gene transfer, primarily from intestinal Epsilon- and Deltaproteobacteria, indicating that horizontal gene transfer has played a key role in defining its niche in the gut ecosystem.
AB - Mucispirillum schaedleri is an abundant inhabitant of the intestinal mucus layer of rodents and other animals and has been suggested to be a pathobiont, a commensal that plays a role in disease. In order to gain insights into its lifestyle, we analyzed the genome and transcriptome of M. schaedleri ASF 457 and performed physiological experiments to test traits predicted by its genome. Although described as a mucus inhabitant, M. schaedleri has limited capacity for degrading host-derived mucosal glycans and other complex polysaccharides. Additionally, M. schaedleri reduces nitrate and expresses systems for scavenging oxygen and reactive oxygen species in vivo, which may account for its localization close to the mucosal tissue and expansion during inflammation. Also of note, M. schaedleri harbors a type VI secretion system and putative effector proteins and can modify gene expression in mucosal tissue, suggesting intimate interactions with its host and a possible role in inflammation. The M. schaedleri genome has been shaped by extensive horizontal gene transfer, primarily from intestinal Epsilon- and Deltaproteobacteria, indicating that horizontal gene transfer has played a key role in defining its niche in the gut ecosystem.
KW - DNRA
KW - Deferribacteres
KW - Fluorescence in situ hybridization
KW - Gut microbiota
KW - Helicobacter
KW - Metatranscriptomics
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U2 - 10.1128/mSystems.00171-16
DO - 10.1128/mSystems.00171-16
M3 - Article
C2 - 28168224
AN - SCOPUS:85020840374
SN - 2379-5077
VL - 2
JO - mSystems
JF - mSystems
IS - 1
M1 - e00171
ER -