Kinetic measurements of CO and O2 binding by the high molecular weight (ca. 3,000,000) hemoglobin from Lumbricus terrestris reveal that: (1) the high co-operativity is associated with a much lower value for 1′ ("on" constant for CO) than in mammalian hemoglobins and (2) the value of 1′ for the subunits is at least 20 times that for the intact polymer. Co-operativity in O2 binding is also manifested kinetically in the O2 dissociation reaction in the absence and presence of CO as a replacement ligand.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - May 7 1971|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology