Light-dark variation in response to chronic nicotine treatment and the density of hypothalamic α-bungarotoxin receptors

Barbara J. Morley, Laura L. Garner

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Chronic nicotine administration increased locomotor activity during the light, but not the dark, in rats maintained on a 12:12-hr light/dark cycle, but the period and peak of the circadian rhythm (CR) were not affected. In Experiment I, 24 male rats were implanted with battery-operated telemeters and locomotor activity was continuously measured for 10 days before and 10 days after the implantation of osmotic mini-pumps which delivered 0, 0.5, 3.0 or 10 mg/kg/day of (±)-nicotine tartrate. Nicotine increased locomotor activity during the light in a dose-dependent manner. Tolerance to the stimulant effects of nicotine during the light occurred in 5-6 days. To determine if the stimulant properties of nicotine were associated with light as opposed to disruption by the environmental stimuli normally present during the day in our animal facility, a second experiment was conducted in which rats were treated with saline or 10 mg/kg/day (±)-nicotine di(+)hydrate tartrate and maintained on a reversed light/dark cycle. Again nicotine increased activity during the light (21:00-09:00) but not dark (09:00-21:00). In a third experiment, the density of α-bungarotoxin binding sites was found to be significantly decreased when animals were sacrificed at 06:00 in comparison with animals sacrificed at 10:00 and 14:00.

Original languageEnglish (US)
Pages (from-to)239-245
Number of pages7
JournalPharmacology, Biochemistry and Behavior
Volume37
Issue number2
DOIs
StatePublished - Oct 1990

Keywords

  • Circadian rhythms
  • Locomotor activity
  • Nicotine
  • Nicotinic receptors
  • Tolerance
  • α-Bungarotoxin binding sites

ASJC Scopus subject areas

  • Biochemistry
  • Toxicology
  • Pharmacology
  • Clinical Biochemistry
  • Biological Psychiatry
  • Behavioral Neuroscience

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