TY - JOUR
T1 - Light-induced plasticity of synaptic ampa receptor composition in retinal ganglion cells
AU - Jones, Rebecca S.
AU - Carroll, Reed C.
AU - Nawy, Scott
N1 - Funding Information:
This research was supported by NEI grant EY017428 (S.N.) and an unrestricted grant from Research to Prevent Blindness (S.N.). R.S.J. performed all experiments and analyzed data. R.S.J., R.C.C., and S.N. conceptualized the study, designed experiments, and discussed results and implications. R.S.J., R.C.C., and S.N. wrote the manuscript.
PY - 2012/8/9
Y1 - 2012/8/9
N2 - Light-evoked responses of all three major classes of retinal ganglion cells (RGCs) are mediated by NMDA receptors (NMDARs) and AMPA receptors (AMPARs). Although synaptic activity at RGC synapses is highly dynamic, synaptic plasticity has not been observed in adult RGCs. Here, using patch-clamp recordings in dark-adapted mouse retina, we report a retina-specific form of AMPAR plasticity. Both chemical and light activation of NMDARs caused the selective endocytosis of GluA2-containing, Ca2+-impermeable AMPARs on RGCs and replacement with GluA2-lacking, Ca2+-permeable AMPARs. The plasticity was expressed in ON but not OFF RGCs and was restricted solely to the ON responses in ON-OFF RGCs. Finally, the plasticity resulted in a shift in the light responsiveness of ON RGCs. Thus, physiologically relevant light stimuli can induce a change in synaptic receptor composition of ON RGCs, providing a mechanism by which the sensitivity of RGC responses may be modified under scotopic conditions.
AB - Light-evoked responses of all three major classes of retinal ganglion cells (RGCs) are mediated by NMDA receptors (NMDARs) and AMPA receptors (AMPARs). Although synaptic activity at RGC synapses is highly dynamic, synaptic plasticity has not been observed in adult RGCs. Here, using patch-clamp recordings in dark-adapted mouse retina, we report a retina-specific form of AMPAR plasticity. Both chemical and light activation of NMDARs caused the selective endocytosis of GluA2-containing, Ca2+-impermeable AMPARs on RGCs and replacement with GluA2-lacking, Ca2+-permeable AMPARs. The plasticity was expressed in ON but not OFF RGCs and was restricted solely to the ON responses in ON-OFF RGCs. Finally, the plasticity resulted in a shift in the light responsiveness of ON RGCs. Thus, physiologically relevant light stimuli can induce a change in synaptic receptor composition of ON RGCs, providing a mechanism by which the sensitivity of RGC responses may be modified under scotopic conditions.
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U2 - 10.1016/j.neuron.2012.05.030
DO - 10.1016/j.neuron.2012.05.030
M3 - Article
C2 - 22884330
AN - SCOPUS:84864934204
SN - 0896-6273
VL - 75
SP - 467
EP - 478
JO - Neuron
JF - Neuron
IS - 3
ER -