TY - JOUR
T1 - Linkage disequilibrium in Brazilian Santa Inês breed, Ovis aries
AU - Alvarenga, Amanda Botelho
AU - Rovadoscki, Gregori Alberto
AU - Petrini, Juliana
AU - Coutinho, Luiz Lehmann
AU - Morota, Gota
AU - Spangler, Matthew L.
AU - Pinto, Luís Fernando Batista
AU - Carvalho, Gleidson Giordano Pinto
AU - Mourão, Gerson Barreto
N1 - Funding Information:
This work was supported by São Paulo Research Foundation (FAPESP-Fundação de Amparo à Pesquisa do Estado de São Paulo; process: 2015/25024-5 and 13/04504-3), Brazil. G.B.M. is recipient of productivity fellowship from CNPq. We are indebted to the Federal University of Bahia (UFBA, Brazil) for the partnership to sheep production and Biotechnology Lab (ESALQ-USP, Brazil) for support in genotyping.
PY - 2018/12/1
Y1 - 2018/12/1
N2 - For genomic selection to be successful, there must be sufficient linkage disequilibrium between the markers and the causal mutations. The objectives of this study were to evaluate the extent of LD in ovine using the Santa Inês breed and to infer the minimum number of markers required to reach reasonable prediction accuracy. In total, 38,168 SNPs and 395 samples were used. The mean LD between adjacent marker pairs measured by r2 and |D′| were 0.166 and 0.617, respectively. LD values between adjacent marker pairs ranged from 0.135 to 0.194 and from 0.568 to 0.650 for r2 for |D′| across all chromosomes. The average r2 between all pairwise SNPs on each chromosome was 0.018. SNPs separated by between 0.10 to 0.20 Mb had an estimated average r2 equal to 0.1033. The identified haplotype blocks consisted of 2 to 21 markers. Moreover, estimates of average coefficients of inbreeding and effective population size were 0.04 and 96, respectively. LD estimated in this study was lower than that reported in other species and was characterized by short haplotype blocks. Our results suggest that the use of a higher density SNP panel is recommended for the implementation of genomic selection in the Santa Inês breed.
AB - For genomic selection to be successful, there must be sufficient linkage disequilibrium between the markers and the causal mutations. The objectives of this study were to evaluate the extent of LD in ovine using the Santa Inês breed and to infer the minimum number of markers required to reach reasonable prediction accuracy. In total, 38,168 SNPs and 395 samples were used. The mean LD between adjacent marker pairs measured by r2 and |D′| were 0.166 and 0.617, respectively. LD values between adjacent marker pairs ranged from 0.135 to 0.194 and from 0.568 to 0.650 for r2 for |D′| across all chromosomes. The average r2 between all pairwise SNPs on each chromosome was 0.018. SNPs separated by between 0.10 to 0.20 Mb had an estimated average r2 equal to 0.1033. The identified haplotype blocks consisted of 2 to 21 markers. Moreover, estimates of average coefficients of inbreeding and effective population size were 0.04 and 96, respectively. LD estimated in this study was lower than that reported in other species and was characterized by short haplotype blocks. Our results suggest that the use of a higher density SNP panel is recommended for the implementation of genomic selection in the Santa Inês breed.
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U2 - 10.1038/s41598-018-27259-7
DO - 10.1038/s41598-018-27259-7
M3 - Article
C2 - 29892085
AN - SCOPUS:85048331357
VL - 8
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 8851
ER -