TY - JOUR
T1 - Lipid and phospholipid profiling of biological samples using MALDI fourier transform mass spectrometry
AU - Batoy, S. Mariccor A.B.
AU - Borgmann, Sabine
AU - Flick, Karin
AU - Griffith, Josephine
AU - Jones, Jeffrey J.
AU - Saraswathi, Viswanathan
AU - Hasty, Alyssa H.
AU - Kaiser, Peter
AU - Wilkins, Charles L.
N1 - Funding Information:
Fig. 4 Histogram plot of lipid and phospholipids found in wild type (top) and met4∆ mutant (bottom) S. cerevisiae lysates Acknowledgments The authors are grateful for financial support from the Arkansas Biosciences Institute and NSF grants CHE-00-91868 and CHE-99-82045. P.K. acknowledges support from NIH-GM-66164. The authors appreciate discussions with R. M. O’Brien and support from the Arkansas Statewide Mass Spectrometry Facility.
PY - 2009/4
Y1 - 2009/4
N2 - Here we describe a study of the feasibility of lipid and phospholipid (PL) profiling using matrix assisted laser desorption/ionization (MALDI) Fourier transform mass spectrometry (FTMS) for two different applications. In this work PL profiles of different mammalian tissues as well as those of whole cell organisms were examined. In particular, comparative analysis of lipid and PL profiles of tissues from mice fed different diets was done and, in another application, MALDI FTMS was used to analyze PL profiles of genetically modified Saccharomyces cerevisiae. Computational sorting of the observed ions was done in order to group the lipid and PL ions from complex MALDI spectra. The PL profiles of liver tissues from mice fed different diets showed a cross correlation coefficient of 0.2580, indicating significant dissimilarity, and revealed more than 30 significantly different peaks at the 99.9% confidence level. Histogram plots derived from the spectra of wild type and genetically modified yeast resulted in a cross correlation coefficient 0.8941 showing greater similarity, but still revealing a number of significantly different peaks. Based on these results, it appears possible to use MALDI FTMS to identify PLs as potential biomarkers for metabolic processes in whole cells and tissues.
AB - Here we describe a study of the feasibility of lipid and phospholipid (PL) profiling using matrix assisted laser desorption/ionization (MALDI) Fourier transform mass spectrometry (FTMS) for two different applications. In this work PL profiles of different mammalian tissues as well as those of whole cell organisms were examined. In particular, comparative analysis of lipid and PL profiles of tissues from mice fed different diets was done and, in another application, MALDI FTMS was used to analyze PL profiles of genetically modified Saccharomyces cerevisiae. Computational sorting of the observed ions was done in order to group the lipid and PL ions from complex MALDI spectra. The PL profiles of liver tissues from mice fed different diets showed a cross correlation coefficient of 0.2580, indicating significant dissimilarity, and revealed more than 30 significantly different peaks at the 99.9% confidence level. Histogram plots derived from the spectra of wild type and genetically modified yeast resulted in a cross correlation coefficient 0.8941 showing greater similarity, but still revealing a number of significantly different peaks. Based on these results, it appears possible to use MALDI FTMS to identify PLs as potential biomarkers for metabolic processes in whole cells and tissues.
KW - Fourier transform mass spectrometry (FTMS)
KW - High fat diet
KW - Matrix assisted laser desorption/ionization mass spectrometry (MALDI)
KW - Saccharomyces cerevisiae
KW - Yeast transcription factor Met4
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U2 - 10.1007/s11745-008-3260-8
DO - 10.1007/s11745-008-3260-8
M3 - Article
C2 - 19005715
AN - SCOPUS:63349089055
VL - 44
SP - 367
EP - 371
JO - Lipids
JF - Lipids
SN - 0024-4201
IS - 4
ER -