Lipid and phospholipid profiling of biological samples using MALDI fourier transform mass spectrometry

S. Mariccor A.B. Batoy, Sabine Borgmann, Karin Flick, Josephine Griffith, Jeffrey J. Jones, Viswanathan Saraswathi, Alyssa H. Hasty, Peter Kaiser, Charles L. Wilkins

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Here we describe a study of the feasibility of lipid and phospholipid (PL) profiling using matrix assisted laser desorption/ionization (MALDI) Fourier transform mass spectrometry (FTMS) for two different applications. In this work PL profiles of different mammalian tissues as well as those of whole cell organisms were examined. In particular, comparative analysis of lipid and PL profiles of tissues from mice fed different diets was done and, in another application, MALDI FTMS was used to analyze PL profiles of genetically modified Saccharomyces cerevisiae. Computational sorting of the observed ions was done in order to group the lipid and PL ions from complex MALDI spectra. The PL profiles of liver tissues from mice fed different diets showed a cross correlation coefficient of 0.2580, indicating significant dissimilarity, and revealed more than 30 significantly different peaks at the 99.9% confidence level. Histogram plots derived from the spectra of wild type and genetically modified yeast resulted in a cross correlation coefficient 0.8941 showing greater similarity, but still revealing a number of significantly different peaks. Based on these results, it appears possible to use MALDI FTMS to identify PLs as potential biomarkers for metabolic processes in whole cells and tissues.

Original languageEnglish (US)
Pages (from-to)367-371
Number of pages5
JournalLipids
Volume44
Issue number4
DOIs
StatePublished - Apr 1 2009

Keywords

  • Fourier transform mass spectrometry (FTMS)
  • High fat diet
  • Matrix assisted laser desorption/ionization mass spectrometry (MALDI)
  • Saccharomyces cerevisiae
  • Yeast transcription factor Met4

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

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    Batoy, S. M. A. B., Borgmann, S., Flick, K., Griffith, J., Jones, J. J., Saraswathi, V., Hasty, A. H., Kaiser, P., & Wilkins, C. L. (2009). Lipid and phospholipid profiling of biological samples using MALDI fourier transform mass spectrometry. Lipids, 44(4), 367-371. https://doi.org/10.1007/s11745-008-3260-8