TY - JOUR
T1 - Liquid chromatography-mass spectrometry method for the analysis of the anti-cancer agent capecitabine and its nucleoside metabolites in human plasma
AU - Xu, Yan
AU - Grem, Jean L.
N1 - Funding Information:
Dr. Xu’s 1-year sabbatical at the National Cancer Institute was funded through an Interagency Personnel Agreement between the National Cancer Institute and Cleveland State University.
PY - 2003/1/5
Y1 - 2003/1/5
N2 - A reversed-phase high-performance liquid chromatography method with electrospray ionization and mass spectral detection is described for the determination of capecitabine, 5′-deoxy-5-fluorocytidine and 5′-deoxy-5-fluorouridine in human plasma with 5-chloro-2′-deoxyuridine as the internal standard. An on-line sample clean-up procedure allows dilution of the plasma sample with the initial mobile phase. The linear dynamic range is 0.0500-10.0 μg/ml for capecitabine, and 0.0500-25.0 μg/ml for the metabolites, 5′-deoxy-5-fluorocytidine and 5′-deoxy-5-fluorouridine, respectively. This method has been used to analyze plasma samples from patients receiving capecitabine in combination with oxaliplatin.
AB - A reversed-phase high-performance liquid chromatography method with electrospray ionization and mass spectral detection is described for the determination of capecitabine, 5′-deoxy-5-fluorocytidine and 5′-deoxy-5-fluorouridine in human plasma with 5-chloro-2′-deoxyuridine as the internal standard. An on-line sample clean-up procedure allows dilution of the plasma sample with the initial mobile phase. The linear dynamic range is 0.0500-10.0 μg/ml for capecitabine, and 0.0500-25.0 μg/ml for the metabolites, 5′-deoxy-5-fluorocytidine and 5′-deoxy-5-fluorouridine, respectively. This method has been used to analyze plasma samples from patients receiving capecitabine in combination with oxaliplatin.
KW - 5-Deoxy-5-fluorocytidine
KW - 5-Deoxy-5-fluorouridine
KW - Capecitabine
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U2 - 10.1016/S1570-0232(02)00674-8
DO - 10.1016/S1570-0232(02)00674-8
M3 - Article
C2 - 12450548
AN - SCOPUS:0037419491
VL - 783
SP - 273
EP - 285
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 1
ER -