Abstract
Listeria monocytogenes transported glucose by a high-affinity phosphoenolpyruvate-dependent phosphotransferase system and a low-affinity proton motive force-mediated system. The low-affinity system (K(m) = 2.9 mM) was inhibited by 2-deoxyglucose and 6-deoxyglucose, whereas the high-affinity system (K(m) = 0.11 mM) was inhibited by 2-deoxyglucose and mannose but not 6-deoxyglucose. Cells and vesicles artificially energized with valinomycin transported glucose or 2-deoxyglucose at rates greater than those of de- energized cells, indicating that a membrane potential could drive uptake by the low-affinity system.
Original language | English (US) |
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Pages (from-to) | 543-546 |
Number of pages | 4 |
Journal | Applied and environmental microbiology |
Volume | 63 |
Issue number | 2 |
DOIs | |
State | Published - Feb 1997 |
ASJC Scopus subject areas
- Biotechnology
- Food Science
- Applied Microbiology and Biotechnology
- Ecology