TY - JOUR
T1 - Liver proteome response of largemouth bass (Micropterus salmoides) exposed to several environmental contaminants
T2 - Potential insights into biomarker development
AU - Sanchez, Brian C.
AU - Ralston-Hooper, Kimberly J.
AU - Kowalski, Kevin A.
AU - Dorota Inerowicz, H.
AU - Adamec, Jiri
AU - Sepúlveda, María S.
N1 - Funding Information:
This work was partially funded by Purdue University Center for the Environment and a Graduate Assistance in Areas of National Need (GAANN) fellowship awarded to the primary author by the US Department of Education. We would like to thank EcoArray (Gainesville, FL) for having funded a parallel study that was used as the source of liver samples for this study. We also thank Nancy Szabo (Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL) for help with tissue chemical analyses.
PY - 2009/10/19
Y1 - 2009/10/19
N2 - Liver proteome response of largemouth bass (Micropterus salmoides) exposed to environmental contaminants was analyzed to identify novel biomarkers of exposure. Adult male bass were exposed to cadmium chloride (CdCl2), atrazine, PCB 126, phenanthrene, or toxaphene via intraperitoneal injection with target body burdens of 0.00067, 3.0, 2.5, 50, and 100 μg/g, respectively. After a 96 h exposure, hepatic proteins were separated with two-dimensional gel electrophoresis and differentially expressed proteins (vs. controls) recognized and identified with MALDI-TOF/TOF mass spectrometry. We identified, 30, 18, eight, 19, and five proteins as differentially expressed within the CdCl2, atrazine, PCB 126, phenanthrene, and toxaphene treatments, respectively. Alterations were observed in the expression of proteins associated with cellular ion homeostasis (toxaphene), oxidative stress (phenanthrene, PCB 126), and energy production including glycolysis (CdCl2, atrazine) and ATP synthesis (atrazine). This work supports the further evaluation of several of these proteins as biomarkers of contaminant exposure in fish.
AB - Liver proteome response of largemouth bass (Micropterus salmoides) exposed to environmental contaminants was analyzed to identify novel biomarkers of exposure. Adult male bass were exposed to cadmium chloride (CdCl2), atrazine, PCB 126, phenanthrene, or toxaphene via intraperitoneal injection with target body burdens of 0.00067, 3.0, 2.5, 50, and 100 μg/g, respectively. After a 96 h exposure, hepatic proteins were separated with two-dimensional gel electrophoresis and differentially expressed proteins (vs. controls) recognized and identified with MALDI-TOF/TOF mass spectrometry. We identified, 30, 18, eight, 19, and five proteins as differentially expressed within the CdCl2, atrazine, PCB 126, phenanthrene, and toxaphene treatments, respectively. Alterations were observed in the expression of proteins associated with cellular ion homeostasis (toxaphene), oxidative stress (phenanthrene, PCB 126), and energy production including glycolysis (CdCl2, atrazine) and ATP synthesis (atrazine). This work supports the further evaluation of several of these proteins as biomarkers of contaminant exposure in fish.
KW - Atrazine
KW - Biomarkers
KW - Cadmium
KW - Largemouth bass
KW - Micropterus salmoides
KW - Proteomics
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U2 - 10.1016/j.aquatox.2009.08.002
DO - 10.1016/j.aquatox.2009.08.002
M3 - Article
C2 - 19717195
AN - SCOPUS:71749121372
SN - 0166-445X
VL - 95
SP - 52
EP - 59
JO - Aquatic Toxicology
JF - Aquatic Toxicology
IS - 1
ER -