LN-3: a diagnostic adjunct in cutaneous graft-versus-host disease.

M. S. Synovec, S. W. Braddock, J. Jones, J. Linder

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Abstract

Expression of HLA Class II antigen on keratinocytes has been advocated as a diagnostic marker of acute graft-versus-host disease (GVHD). LN-3 is a murine monoclonal antibody marking an HLA Class II antigen that survives formalin fixation and paraffin embedding. We analyzed the LN-3 staining pattern on 56 skin biopsies from patients treated with bone marrow or peripheral stem cell transplantation, non-transplant patients receiving conventional chemotherapy and/or radiation therapy and controls. Keratinocyte staining, endothelial staining, and the number of epidermal and dermal Langerhans cells were evaluated and analyzed with respect to the histologic and clinical diagnosis in a blind and retrospective fashion. The most predictive parameter for GVHD was marking of endothelial cells by LN-3 which was present in 12 of 16 (75%) biopsies from patients with GVHD. Endothelial staining was found in a total of 19 biopsies of which 12 (63%) had GVHD. These data suggest that LN-3 immunohistochemistry may help identify cutaneous GVHD and discriminate it from conditions that are histologically similar.

Original languageEnglish (US)
Pages (from-to)643-647
Number of pages5
JournalModern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
Volume3
Issue number6
StatePublished - Nov 1990

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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