Localization of CD8 T cell epitope within cardiac myosin heavy chain-α334-352 that induces autoimmune myocarditis in A/J mice

Chandirasegaran Massilamany, Arunakumar Gangaplara, Rakesh H. Basavalingappa, Rajkumar A. Rajasekaran, Vahid Khalilzad-Sharghi, Zhongji Han, Shadi Othman, David Steffen, Jay Reddy

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Background: Cardiac myosin heavy chain-α (Myhc), an intracellular protein expressed in the cardiomyocytes, has been identified as a major autoantigen in cardiac autoimmunity. In our studies with Myhc334-352-induced experimental autoimmune myocarditis in A/J mice (H-2ª), we discovered that Myhc334-352, supposedly a CD4 T cell epitope, also induced antigen-specific CD8 T cells that transfer disease to naive animals. Methods and results: In our efforts to identify the CD8 T cell determinants, we localized Myhc338-348 within the full length-Myhc334-352, leading to four key findings. (1) By acting as a dual epitope, Myhc338-348 induces both CD4 and CD8 T cell responses. (2) In a major histocompatibility complex (MHC) class I-stabilization assay, Myhc338-348 was found to bind H-2Dd - but not H-2Kk or H-2Ld - alleles. (3) The CD8 T cell response induced by Myhc338-348 was antigen-specific, as evaluated by MHC class I/H-2Dd dextramer staining. The antigen-sensitized T cells predominantly produced interferon-γ, the critical cytokine of effector cytotoxic T lymphocytes. (4) Myhc338-348 was found to induce myocarditis in immunized animals as determined by histology and magnetic resonance microscopy imaging. Conclusions: Our data provide new insights as to how different immune cells can recognize the same antigen and inflict damage through different mechanisms.

Original languageEnglish (US)
Pages (from-to)311-321
Number of pages11
JournalInternational Journal of Cardiology
Volume202
DOIs
StatePublished - Jan 1 2016

Keywords

  • Autoimmunity
  • CD8 T cells
  • Cardiac myosin
  • MHC class I dextramers
  • Myocarditis

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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