Abstract
The objective of this study was to determine whether progesterone-induced estrogen receptor regulatory factor (ReRF) is a component of hamster uterine nuclei or a cytpplasmic contaminant of the nuclear fraction. Crude uterine nuclei were prepared in low salt buffer and were purified by either the hexylene glycol method or an isotonic sucrose-Triton X-100 procedure. ReRF activity was measured as a net percentage increase in estrogen receptor inactivation after incubation of a 0.5 M KCl nuclear extract at 36°C for 30 min. Progesterone treatment (5 mg/kg s.c. in oil, 2 h) increased net receptor inactivation to the same extent (9-12% net increase) in extract made from each nuclear preparation. Extensive washing of nuclei caused a progressive decline in recovery of ReRF activity, in parallel with the behavior of KCl-extractable nuclear protein and estrogen receptor. These results demonstrate that ReRF is a loosely bound component of the uterine nucleus.
Original language | English (US) |
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Pages (from-to) | 301-313 |
Number of pages | 13 |
Journal | Molecular and Cellular Endocrinology |
Volume | 32 |
Issue number | 2-3 |
DOIs | |
State | Published - Oct 1983 |
Externally published | Yes |
Keywords
- estrogen receptor
- hamster
- progesterone induction
- receptor inactivation
- uterus
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Endocrinology