Localization of estrogen receptor regulatory factor in the uterine nucleus

R. G. MacDonald, S. P. Rosenberg, W. W. Leavitt

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The objective of this study was to determine whether progesterone-induced estrogen receptor regulatory factor (ReRF) is a component of hamster uterine nuclei or a cytpplasmic contaminant of the nuclear fraction. Crude uterine nuclei were prepared in low salt buffer and were purified by either the hexylene glycol method or an isotonic sucrose-Triton X-100 procedure. ReRF activity was measured as a net percentage increase in estrogen receptor inactivation after incubation of a 0.5 M KCl nuclear extract at 36°C for 30 min. Progesterone treatment (5 mg/kg s.c. in oil, 2 h) increased net receptor inactivation to the same extent (9-12% net increase) in extract made from each nuclear preparation. Extensive washing of nuclei caused a progressive decline in recovery of ReRF activity, in parallel with the behavior of KCl-extractable nuclear protein and estrogen receptor. These results demonstrate that ReRF is a loosely bound component of the uterine nucleus.

Original languageEnglish (US)
Pages (from-to)301-313
Number of pages13
JournalMolecular and Cellular Endocrinology
Volume32
Issue number2-3
DOIs
StatePublished - Oct 1983
Externally publishedYes

Keywords

  • estrogen receptor
  • hamster
  • progesterone induction
  • receptor inactivation
  • uterus

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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