Localization of low-melting regions in phage T7 DNA

V. I. Lyamichev; I. G. Panyutin; D. I. Cherny; Yu L. Lyubchenko

doi:10.1093/nar/11.7.2165

Localization of low-melting regions in phage T7 DNA

V. I. Lyamichev, I. G. Panyutin, D. I. Cherny, Yu L. Lyubchenko

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Specific fragmentation of T7 DNA at glyoxal-fixed denatured regions by the S1 endonuclease followed by restriction analysis made it possible to localize four low-melting regions in phage T7 DNA. These regions have the following coordinates:0.5-1.2;14.8±0.3;46.3±0.5; 98.4±0.3 (in T7 DNA length units). The location of the low-melting regions was refined by means of electron-microscopic denaturation mapping and gel electrophoresis of partially denatured DNA. The obtained localization of the low-melting regions is consistent with the available data on the sequence of T7 DNA. The map of low-melting regions was compared with the genetic map of T7 DNA.

Original language	English (US)
Pages (from-to)	2165-2176
Number of pages	12
Journal	Nucleic acids research
Volume	11
Issue number	7
DOIs	https://doi.org/10.1093/nar/11.7.2165
State	Published - Apr 11 1983
Externally published	Yes

ASJC Scopus subject areas

Genetics

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title = "Localization of low-melting regions in phage T7 DNA",

abstract = "Specific fragmentation of T7 DNA at glyoxal-fixed denatured regions by the S1 endonuclease followed by restriction analysis made it possible to localize four low-melting regions in phage T7 DNA. These regions have the following coordinates:0.5-1.2;14.8±0.3;46.3±0.5; 98.4±0.3 (in T7 DNA length units). The location of the low-melting regions was refined by means of electron-microscopic denaturation mapping and gel electrophoresis of partially denatured DNA. The obtained localization of the low-melting regions is consistent with the available data on the sequence of T7 DNA. The map of low-melting regions was compared with the genetic map of T7 DNA.",

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T1 - Localization of low-melting regions in phage T7 DNA

AU - Lyamichev, V. I.

AU - Panyutin, I. G.

AU - Cherny, D. I.

AU - Lyubchenko, Yu L.

PY - 1983/4/11

Y1 - 1983/4/11

N2 - Specific fragmentation of T7 DNA at glyoxal-fixed denatured regions by the S1 endonuclease followed by restriction analysis made it possible to localize four low-melting regions in phage T7 DNA. These regions have the following coordinates:0.5-1.2;14.8±0.3;46.3±0.5; 98.4±0.3 (in T7 DNA length units). The location of the low-melting regions was refined by means of electron-microscopic denaturation mapping and gel electrophoresis of partially denatured DNA. The obtained localization of the low-melting regions is consistent with the available data on the sequence of T7 DNA. The map of low-melting regions was compared with the genetic map of T7 DNA.

AB - Specific fragmentation of T7 DNA at glyoxal-fixed denatured regions by the S1 endonuclease followed by restriction analysis made it possible to localize four low-melting regions in phage T7 DNA. These regions have the following coordinates:0.5-1.2;14.8±0.3;46.3±0.5; 98.4±0.3 (in T7 DNA length units). The location of the low-melting regions was refined by means of electron-microscopic denaturation mapping and gel electrophoresis of partially denatured DNA. The obtained localization of the low-melting regions is consistent with the available data on the sequence of T7 DNA. The map of low-melting regions was compared with the genetic map of T7 DNA.

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Localization of low-melting regions in phage T7 DNA

Abstract

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