Localization of melted regions in supercoiled DNA

O. N. Voloshin; L. S. Shlyakhtenko; Yu L. Lyubchenko

doi:10.1016/0014-5793(89)80165-6

Localization of melted regions in supercoiled DNA

O. N. Voloshin, L. S. Shlyakhtenko, Yu L. Lyubchenko

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11 Scopus citations

Abstract

Diethyl pyrocarbonate (DEPC) was used as a probe of local denatured regions in ccDNA pAO3 plasmid. It was found that in native ccDNA molecules only adenosine residues in the loop of the cruciform structure react with DEPC. Denaturation of ccDNA is accompanied by the appearance of two short regions (20 bp long) at both borders of the cruciform structure. Further increase in the denaturation process is associated with considerable expansion of the region located to the left of the cruciform, while the cruciform structure itself and the denatured region located to the right of it disappear.

Original language	English (US)
Pages (from-to)	377-380
Number of pages	4
Journal	FEBS Letters
Volume	243
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(89)80165-6
State	Published - Jan 30 1989
Externally published	Yes

Keywords

Cruciform structure
DNA modification
DNA, supercoiled
Melting pattern
Open region

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(89)80165-6

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title = "Localization of melted regions in supercoiled DNA",

abstract = "Diethyl pyrocarbonate (DEPC) was used as a probe of local denatured regions in ccDNA pAO3 plasmid. It was found that in native ccDNA molecules only adenosine residues in the loop of the cruciform structure react with DEPC. Denaturation of ccDNA is accompanied by the appearance of two short regions (20 bp long) at both borders of the cruciform structure. Further increase in the denaturation process is associated with considerable expansion of the region located to the left of the cruciform, while the cruciform structure itself and the denatured region located to the right of it disappear.",

keywords = "Cruciform structure, DNA modification, DNA, supercoiled, Melting pattern, Open region",

author = "Voloshin, {O. N.} and Shlyakhtenko, {L. S.} and Lyubchenko, {Yu L.}",

year = "1989",

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day = "30",

doi = "10.1016/0014-5793(89)80165-6",

language = "English (US)",

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T1 - Localization of melted regions in supercoiled DNA

AU - Voloshin, O. N.

AU - Shlyakhtenko, L. S.

AU - Lyubchenko, Yu L.

PY - 1989/1/30

Y1 - 1989/1/30

N2 - Diethyl pyrocarbonate (DEPC) was used as a probe of local denatured regions in ccDNA pAO3 plasmid. It was found that in native ccDNA molecules only adenosine residues in the loop of the cruciform structure react with DEPC. Denaturation of ccDNA is accompanied by the appearance of two short regions (20 bp long) at both borders of the cruciform structure. Further increase in the denaturation process is associated with considerable expansion of the region located to the left of the cruciform, while the cruciform structure itself and the denatured region located to the right of it disappear.

AB - Diethyl pyrocarbonate (DEPC) was used as a probe of local denatured regions in ccDNA pAO3 plasmid. It was found that in native ccDNA molecules only adenosine residues in the loop of the cruciform structure react with DEPC. Denaturation of ccDNA is accompanied by the appearance of two short regions (20 bp long) at both borders of the cruciform structure. Further increase in the denaturation process is associated with considerable expansion of the region located to the left of the cruciform, while the cruciform structure itself and the denatured region located to the right of it disappear.

KW - Cruciform structure

KW - DNA modification

KW - DNA, supercoiled

KW - Melting pattern

KW - Open region

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DO - 10.1016/0014-5793(89)80165-6

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SP - 377

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JO - FEBS Letters

JF - FEBS Letters

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ER -

Localization of melted regions in supercoiled DNA

Abstract

Keywords

ASJC Scopus subject areas

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