Localization of melted regions in supercoiled DNA

O. N. Voloshin, L. S. Shlyakhtenko, Yu L. Lyubchenko

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Diethyl pyrocarbonate (DEPC) was used as a probe of local denatured regions in ccDNA pAO3 plasmid. It was found that in native ccDNA molecules only adenosine residues in the loop of the cruciform structure react with DEPC. Denaturation of ccDNA is accompanied by the appearance of two short regions (20 bp long) at both borders of the cruciform structure. Further increase in the denaturation process is associated with considerable expansion of the region located to the left of the cruciform, while the cruciform structure itself and the denatured region located to the right of it disappear.

Original languageEnglish (US)
Pages (from-to)377-380
Number of pages4
JournalFEBS Letters
Volume243
Issue number2
DOIs
StatePublished - Jan 30 1989
Externally publishedYes

Keywords

  • Cruciform structure
  • DNA modification
  • DNA, supercoiled
  • Melting pattern
  • Open region

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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