Localization of [3H]cyclohexyladenosine and [3H]nitrobenzylthionosine binding sites in rat striatum and superior colliculus

Jonathan D. Geiger

doi:10.1016/0006-8993(86)91033-4

Localization of [³H]cyclohexyladenosine and [³H]nitrobenzylthionosine binding sites in rat striatum and superior colliculus

Jonathan D. Geiger

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

The localization of adenosine receptors labelled with [³H]cyclohexyladenosine ([³H]CHA) and adenosine transport sites labelled with [³H]nitrobenzylthioinosine ([³H]NBI) was examined in striatum and superior colliculus (SC) using radioligand binding and lesioning methods. Striatal kainic acid lesions significantly reduced the number (B_max) of a single class of high affinity binding sites for [³H]CHA by 50% and that for [³H]NBI by 15% without altering K_d values for either ligand. In SC, enucleations significantly reduced both high and low affinity [³H]CHA binding sites by about 60% while levels of [³H]NBI binding were unaffected. Thus, adenosine receptors are present on striatal interneurons and retinal projections to the SC and some [³H]NBI binding sites are located on striatal interneurons.

Original language	English (US)
Pages (from-to)	404-408
Number of pages	5
Journal	Brain Research
Volume	363
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(86)91033-4
State	Published - Jan 22 1986
Externally published	Yes

Keywords

adenosine receptor
cyclohexyladenosine
nitrobenzylthioinosine
nucleoside transport site
striatum
superior colliculus

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

Access to Document

10.1016/0006-8993(86)91033-4

Cite this

@article{8c6b9e2209b1466a950bb80d9f8f2946,

title = "Localization of [3H]cyclohexyladenosine and [3H]nitrobenzylthionosine binding sites in rat striatum and superior colliculus",

abstract = "The localization of adenosine receptors labelled with [3H]cyclohexyladenosine ([3H]CHA) and adenosine transport sites labelled with [3H]nitrobenzylthioinosine ([3H]NBI) was examined in striatum and superior colliculus (SC) using radioligand binding and lesioning methods. Striatal kainic acid lesions significantly reduced the number (Bmax) of a single class of high affinity binding sites for [3H]CHA by 50% and that for [3H]NBI by 15% without altering Kd values for either ligand. In SC, enucleations significantly reduced both high and low affinity [3H]CHA binding sites by about 60% while levels of [3H]NBI binding were unaffected. Thus, adenosine receptors are present on striatal interneurons and retinal projections to the SC and some [3H]NBI binding sites are located on striatal interneurons.",

keywords = "adenosine receptor, cyclohexyladenosine, nitrobenzylthioinosine, nucleoside transport site, striatum, superior colliculus",

author = "Geiger, {Jonathan D.}",

note = "Funding Information: I wish to thank Ms. Kosala Sivananthan and Mr. Mark Whittaker for their excellent technical assistance. This work was supported by a grant from the Medical Research Council of Canada (MRC). J.D.G. is a Scholar of the Manitoba Health Research Council.",

year = "1986",

month = jan,

day = "22",

doi = "10.1016/0006-8993(86)91033-4",

language = "English (US)",

volume = "363",

pages = "404--408",

journal = "Brain Research",

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publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Localization of [3H]cyclohexyladenosine and [3H]nitrobenzylthionosine binding sites in rat striatum and superior colliculus

AU - Geiger, Jonathan D.

N1 - Funding Information: I wish to thank Ms. Kosala Sivananthan and Mr. Mark Whittaker for their excellent technical assistance. This work was supported by a grant from the Medical Research Council of Canada (MRC). J.D.G. is a Scholar of the Manitoba Health Research Council.

PY - 1986/1/22

Y1 - 1986/1/22

N2 - The localization of adenosine receptors labelled with [3H]cyclohexyladenosine ([3H]CHA) and adenosine transport sites labelled with [3H]nitrobenzylthioinosine ([3H]NBI) was examined in striatum and superior colliculus (SC) using radioligand binding and lesioning methods. Striatal kainic acid lesions significantly reduced the number (Bmax) of a single class of high affinity binding sites for [3H]CHA by 50% and that for [3H]NBI by 15% without altering Kd values for either ligand. In SC, enucleations significantly reduced both high and low affinity [3H]CHA binding sites by about 60% while levels of [3H]NBI binding were unaffected. Thus, adenosine receptors are present on striatal interneurons and retinal projections to the SC and some [3H]NBI binding sites are located on striatal interneurons.

AB - The localization of adenosine receptors labelled with [3H]cyclohexyladenosine ([3H]CHA) and adenosine transport sites labelled with [3H]nitrobenzylthioinosine ([3H]NBI) was examined in striatum and superior colliculus (SC) using radioligand binding and lesioning methods. Striatal kainic acid lesions significantly reduced the number (Bmax) of a single class of high affinity binding sites for [3H]CHA by 50% and that for [3H]NBI by 15% without altering Kd values for either ligand. In SC, enucleations significantly reduced both high and low affinity [3H]CHA binding sites by about 60% while levels of [3H]NBI binding were unaffected. Thus, adenosine receptors are present on striatal interneurons and retinal projections to the SC and some [3H]NBI binding sites are located on striatal interneurons.

KW - adenosine receptor

KW - cyclohexyladenosine

KW - nitrobenzylthioinosine

KW - nucleoside transport site

KW - striatum

KW - superior colliculus

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