Localization of the human fibronectin (FN) gene on chromosome 8 by a specific enzyme immunoassay

Stephen I. Rennard; Robert L. Church; David H. Rohrbach; Dolores E. Shupp; Shigeto Abe; A. Tyl Hewitt; J. Clifford Murray; George R. Martin

doi:10.1007/BF00484626

Localization of the human fibronectin (FN) gene on chromosome 8 by a specific enzyme immunoassay

Stephen I. Rennard, Robert L. Church, David H. Rohrbach, Dolores E. Shupp, Shigeto Abe, A. Tyl Hewitt, J. Clifford Murray, George R. Martin

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The fibronectin produced by clonal murine-human hybrid cell lines containing various complements of human chromosomes was measured. Human and murine fibronectins were assayed by specific immunoassay, and the production of human fibronectin was correlated with karyology and isozyme markers for specific human chromosomes. The data show a 100% concordance between the expression of human fibronectin and glutathione reductase, a marker for human chromosome 8, indicating that chromosome 8 codes for the fibronectin polypeptide.

Original language	English (US)
Pages (from-to)	551-566
Number of pages	16
Journal	Biochemical Genetics
Volume	19
Issue number	5-6
DOIs	https://doi.org/10.1007/BF00484626
State	Published - Jun 1981
Externally published	Yes

Keywords

ELISA
fibronecting
gene mapping
murine-human hybrids

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Biochemistry
Molecular Biology
Genetics

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10.1007/BF00484626

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Localization of the human fibronectin (FN) gene on chromosome 8 by a specific enzyme immunoassay. / Rennard, Stephen I.; Church, Robert L.; Rohrbach, David H.; Shupp, Dolores E.; Abe, Shigeto; Hewitt, A. Tyl; Murray, J. Clifford; Martin, George R.

In: Biochemical Genetics, Vol. 19, No. 5-6, 06.1981, p. 551-566.

Research output: Contribution to journal › Article › peer-review

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title = "Localization of the human fibronectin (FN) gene on chromosome 8 by a specific enzyme immunoassay",

abstract = "The fibronectin produced by clonal murine-human hybrid cell lines containing various complements of human chromosomes was measured. Human and murine fibronectins were assayed by specific immunoassay, and the production of human fibronectin was correlated with karyology and isozyme markers for specific human chromosomes. The data show a 100% concordance between the expression of human fibronectin and glutathione reductase, a marker for human chromosome 8, indicating that chromosome 8 codes for the fibronectin polypeptide.",

keywords = "ELISA, fibronecting, gene mapping, murine-human hybrids",

author = "Rennard, {Stephen I.} and Church, {Robert L.} and Rohrbach, {David H.} and Shupp, {Dolores E.} and Shigeto Abe and Hewitt, {A. Tyl} and Murray, {J. Clifford} and Martin, {George R.}",

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AU - Rennard, Stephen I.

AU - Church, Robert L.

AU - Rohrbach, David H.

AU - Shupp, Dolores E.

AU - Abe, Shigeto

AU - Hewitt, A. Tyl

AU - Murray, J. Clifford

AU - Martin, George R.

PY - 1981/6

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N2 - The fibronectin produced by clonal murine-human hybrid cell lines containing various complements of human chromosomes was measured. Human and murine fibronectins were assayed by specific immunoassay, and the production of human fibronectin was correlated with karyology and isozyme markers for specific human chromosomes. The data show a 100% concordance between the expression of human fibronectin and glutathione reductase, a marker for human chromosome 8, indicating that chromosome 8 codes for the fibronectin polypeptide.

AB - The fibronectin produced by clonal murine-human hybrid cell lines containing various complements of human chromosomes was measured. Human and murine fibronectins were assayed by specific immunoassay, and the production of human fibronectin was correlated with karyology and isozyme markers for specific human chromosomes. The data show a 100% concordance between the expression of human fibronectin and glutathione reductase, a marker for human chromosome 8, indicating that chromosome 8 codes for the fibronectin polypeptide.

KW - ELISA

KW - fibronecting

KW - gene mapping

KW - murine-human hybrids

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SN - 0006-2928

IS - 5-6

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Localization of the human fibronectin (FN) gene on chromosome 8 by a specific enzyme immunoassay

Abstract

Keywords

ASJC Scopus subject areas

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