Location of T-cell epitopes in nonstructural proteins 9 and 10 of type-II porcine reproductive and respiratory syndrome virus

Rajeshwari Parida, In Soo Choi, Daniel A. Peterson, Asit K. Pattnaik, William Laegreid, Federico A. Zuckermann, Fernando A. Osorio

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant swine pathogen which exhibits considerable sequence diversity. In an attempt to identify highly conserved T-cell epitopes contained in proteins of this virus, we examined heptadecamer peptides spanning the sequence of the PRRSV nonstructural proteins (NSPs) 9 and 10, both of which are highly conserved, for their ability to elicit a recall proliferative and interferon-gamma response in peripheral blood mononuclear cells obtained from pigs immunized against the type-II PRRSV strain FL-12. These studies led to the identification of four peptides, two from each NSP9 and NSP10 that appear to contain T-cell epitopes. Comparison of the amino acid sequence of these four peptide sequences to the analogous sequences from a diverse sample of type-II PRRSV strains indicated that these sequences are highly conserved and thus contain highly conserved T-cell epitopes. The identified epitopes may be important in the formulation of immunogens to provide broad cross-protection against diverse PRRSV strains.

Original languageEnglish (US)
Pages (from-to)13-21
Number of pages9
JournalVirus Research
Volume169
Issue number1
DOIs
StatePublished - Oct 1 2012

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Keywords

  • Nonstructural proteins
  • PRRSV vaccines
  • Porcine reproductive and respiratory syndrome virus
  • T-cell epitopes

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

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