TY - JOUR
T1 - Long-range restriction enzyme maps of DNF15S2, D3S2, and c-raf1 loci on the short arm of human chromosome 3
AU - Boldog, Ferenc
AU - Erlandsson, Rikard
AU - Klein, George
AU - Sumegi, Janos
N1 - Funding Information:
The authors thank Drs. Leder and Ernberg for the cells, Carritt and White for the probes, Barlow and Lehrach for their detailed protocol, and the organizers and participants of the Long Range DNA Mapping EMBO course (Oxford, England, 1988) for their discussions. This investigation was supported by PHS grant 5 R01 CA14054-15 awarded by the National Cancer Institute, DHHS, and by the Swedish Cancer Society. R. E. and S. J. are recipients of fellowships from the Cancer Research Institute and the Concern Foundation. F. B. was supported by the Swedish Cancer Society.
PY - 1989/10/15
Y1 - 1989/10/15
N2 - Physical maps have been constructed around loci DNF15S2, D3S2, and c-raf1 on the short arm of human chromosome 3 using pulsed field gradient gel electrophoresis. The normal restriction pattern has not been altered by a t(3;8)(p14.2;q24.1) characteristic for a hereditary form of renal cell carcinoma, indicating that the breakpoint itself is not included in any of the mapped areas. We have found a CpG island within the DNF15S2 locus, suggesting the presence of a functional gene in the region.
AB - Physical maps have been constructed around loci DNF15S2, D3S2, and c-raf1 on the short arm of human chromosome 3 using pulsed field gradient gel electrophoresis. The normal restriction pattern has not been altered by a t(3;8)(p14.2;q24.1) characteristic for a hereditary form of renal cell carcinoma, indicating that the breakpoint itself is not included in any of the mapped areas. We have found a CpG island within the DNF15S2 locus, suggesting the presence of a functional gene in the region.
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U2 - 10.1016/0165-4608(89)90098-8
DO - 10.1016/0165-4608(89)90098-8
M3 - Article
C2 - 2571406
AN - SCOPUS:0024425614
SN - 0165-4608
VL - 42
SP - 295
EP - 306
JO - Cancer genetics and cytogenetics
JF - Cancer genetics and cytogenetics
IS - 2
ER -