Long-short-long games in mRNA identification: The length matters

San Ming Wang

Research output: Contribution to journalReview articlepeer-review

6 Scopus citations

Abstract

The multiple levels of post-transcriptional processing and million-fold differences in expression levels make fully decoding the transcriptome in any given species extremely challenging. This review addresses the influence of sequenced length on transcriptome decoding under current DNA sequencing capabilities. Comparison of full-length cDNA, EST, 14-base SAGE, 21-LongSAGE, 26-SuperSAGE, and other variations shows that the sequenced length has been a key factor in determining the sensitivity and specificity for mRNA detection.

Original languageEnglish (US)
Pages (from-to)362-367
Number of pages6
JournalCurrent Pharmaceutical Biotechnology
Volume9
Issue number5
DOIs
StatePublished - 2008

Keywords

  • EST
  • Full-length cDNA
  • LongSAGE
  • SAGE
  • SuperSAGE
  • Transcriptome

ASJC Scopus subject areas

  • Biotechnology
  • Pharmaceutical Science

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