TY - JOUR
T1 - Low-temperature crosslinking of proteins using non-toxic citric acid in neutral aqueous medium
T2 - Mechanism and kinetic study
AU - Xu, Helan
AU - Shen, Li
AU - Xu, Lan
AU - Yang, Yiqi
PY - 2015/11/5
Y1 - 2015/11/5
N2 - For the first time, mechanism and kinetics of citric acid crosslinking of protein in neutral aqueous condition under low temperature are reported. Crosslinking was a conventional method to improve properties of protein products to replace petroleum-derived polymers. However, current crosslinking methods were toxic, expensive or required harsh conditions. Non-toxic carboxylic acid could effectively enhance wet performance properties of multiple protein products under mild conditions, which were critical for preserving the activities during immobilization of enzymes. In this study, more than one carboxyl groups in one citric acid molecule were found capable of reacting with protein in aqueous medium at pH 6.8 under 50 and 75°C, verifying occurrence of crosslinking. The pseudo second-order reaction had activation energy as low as 24.956 kJ mol-1, indicating its easy occurrence. Mild crosslinking using citric acid might provide alternative to accelerate low-cost and efficient enzyme immobilization as well as industrial utilization of protein-based products.
AB - For the first time, mechanism and kinetics of citric acid crosslinking of protein in neutral aqueous condition under low temperature are reported. Crosslinking was a conventional method to improve properties of protein products to replace petroleum-derived polymers. However, current crosslinking methods were toxic, expensive or required harsh conditions. Non-toxic carboxylic acid could effectively enhance wet performance properties of multiple protein products under mild conditions, which were critical for preserving the activities during immobilization of enzymes. In this study, more than one carboxyl groups in one citric acid molecule were found capable of reacting with protein in aqueous medium at pH 6.8 under 50 and 75°C, verifying occurrence of crosslinking. The pseudo second-order reaction had activation energy as low as 24.956 kJ mol-1, indicating its easy occurrence. Mild crosslinking using citric acid might provide alternative to accelerate low-cost and efficient enzyme immobilization as well as industrial utilization of protein-based products.
KW - Aqueous medium
KW - Citric acid
KW - Kinetics
KW - Neutral pH
KW - Non-toxic crosslinker
KW - Protein
UR - http://www.scopus.com/inward/record.url?scp=84930205253&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84930205253&partnerID=8YFLogxK
U2 - 10.1016/j.indcrop.2015.05.010
DO - 10.1016/j.indcrop.2015.05.010
M3 - Article
AN - SCOPUS:84930205253
VL - 74
SP - 234
EP - 240
JO - Industrial Crops and Products
JF - Industrial Crops and Products
SN - 0926-6690
ER -