@article{cf983dbca77b46838e36934f4644b404,
title = "Lung transcriptome of nonhuman primates exposed to total- and partial-body irradiation",
abstract = "The focus of radiation biodosimetry has changed recently, and a paradigm shift for using molecular technologies of omic platforms in addition to cytogenetic techniques has been observed. In our study, we have used a nonhuman primate model to investigate the impact of a supralethal dose of 12 Gy radiation on alterations in the lung transcriptome. We used 6 healthy and 32 irradiated animal samples to delineate radiation-induced changes. We also used a medical countermeasure, γ-tocotrienol (GT3), to observe any changes. We demonstrate significant radiation-induced changes in the lung transcriptome for total-body irradiation (TBI) and partial-body irradiation (PBI). However, no major influence of GT3 on radiation was noted in either comparison. Several common signaling pathways, including PI3K/AKT, GADD45, and p53, were upregulated in both exposures. TBI activated DNA-damage-related pathways in the lungs, whereas PTEN signaling was activated after PBI. Our study highlights the various transcriptional profiles associated with γ- and X-ray exposures, and the associated pathways include LXR/RXR activation in TBI, whereas pulmonary wound-healing and pulmonary fibrosis signaling was repressed in PBI. Our study provides important insights into the molecular pathways associated with irradiation that can be further investigated for biomarker discovery.",
keywords = "MT: bioinformatics, lung, nonhuman primates, partial-body irradiation, radiation countermeasure, radiation injury, total-body irradiation, transcriptomics, γ-tocotrienol",
author = "Vellichirammal, {Neetha Nanoth} and Sahil Sethi and Sanjit Pandey and Jatinder Singh and Wise, {Stephen Y.} and Carpenter, {Alana D.} and Fatanmi, {Oluseyi O.} and Chittibabu Guda and Singh, {Vijay K.}",
note = "Funding Information: The authors thank the Bioinformatics and Systems Biology Core ( BSBC ) facility at University of Nebraska Medical Center for providing the computational infrastructure and support. BSBC receives support from Nebraska Research Initiative (NRI) and NIH awards ( 2P20GM103427 , 5P30CA036727 , 2U54GM115458 ). The authors also acknowledge the Holland Computing Center of the University of Nebraska-Lincoln for computational resources, which receives support from the NRI. The opinions or assertions contained herein are the private views of the authors and are not necessarily those of the Uniformed Services University of the Health Sciences or the Department of Defense, USA. Funding Information: The authors thank the Bioinformatics and Systems Biology Core (BSBC) facility at University of Nebraska Medical Center for providing the computational infrastructure and support. BSBC receives support from Nebraska Research Initiative (NRI) and NIH awards (2P20GM103427, 5P30CA036727, 2U54GM115458). The authors also acknowledge the Holland Computing Center of the University of Nebraska-Lincoln for computational resources, which receives support from the NRI. The opinions or assertions contained herein are the private views of the authors and are not necessarily those of the Uniformed Services University of the Health Sciences or the Department of Defense, USA. Study design, V.K.S.; performance of the study, V.K.S. S.Y.W. A.D.C. and O.O.F.; transcriptomic data acquisition, J.S.; transcriptomic data analysis, N.N.V. and S.P.; drafting of the manuscript, V.K.S. S.Y.W. A.D.C. N.N.V. S.S. and C.G.; revision of manuscript content, VK..S. N.N.V. and C.G.; funding acquisition, V.K.S. All authors have read and approved the final submitted manuscript. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2022",
year = "2022",
month = sep,
day = "13",
doi = "10.1016/j.omtn.2022.08.006",
language = "English (US)",
volume = "29",
pages = "584--598",
journal = "Molecular Therapy - Nucleic Acids",
issn = "2162-2531",
publisher = "Nature Publishing Group",
}