Lymphocytes bearing receptors for both sheep erythrocytes and complement in patients with neoplastic and non-neoplastic diseases

Kazimiera J. Gajl-Peczalska; Sandra Chartrand; Clara D. Bloomfield; Jean Corte; Peter F. Coccia; Mark E. Nesbit; John H. Kersey

doi:10.1016/0090-1229(77)90119-2

Lymphocytes bearing receptors for both sheep erythrocytes and complement in patients with neoplastic and non-neoplastic diseases

Kazimiera J. Gajl-Peczalska, Sandra Chartrand, Clara D. Bloomfield, Jean Corte, Peter F. Coccia, Mark E. Nesbit, John H. Kersey

Pediatrics Hematology/Oncology

Research output: Contribution to journal › Article › peer-review

Abstract

To identify lymphoid cells bearing receptors for both sheep erythrocytes (sE) and complement (C′), a combined rosette assay was developed using uncoated sE and chicken erythrocytes coated with antibody and complement (cEAC′). Cells with both receptors were present in low percentages in the blood of normal individuals. Tissues and/or blood from 543 patients with various diseases were studied using the combined rosette assay. Large numbers of malignant cells were found to carry receptors for both C′ and sE in four patients with lymphoproliferative malignancies. Two were children with lymphoma who had mediastinal masses. The other two were adults with chronic lymphocytic leukemia; their cells also carried surface immunoglobulin and Fc receptors. In these four patients double or multiple receptor lymphocytes may represent a clonal proliferation of subsets of lymphocytes present in small numbers under normal conditions. An increase in double receptor lymphocytes was rare in the non-neoplastic conditions studied and seen only in children. Double receptor lymphocytes may represent an immature or "stem" cell lymphocyte subset.

Original language	English (US)
Pages (from-to)	292-299
Number of pages	8
Journal	Clinical Immunology and Immunopathology
Volume	8
Issue number	2
DOIs	https://doi.org/10.1016/0090-1229(77)90119-2
State	Published - Sep 1977

ASJC Scopus subject areas

Immunology and Allergy
Pathology and Forensic Medicine
Immunology

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10.1016/0090-1229(77)90119-2

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title = "Lymphocytes bearing receptors for both sheep erythrocytes and complement in patients with neoplastic and non-neoplastic diseases",

abstract = "To identify lymphoid cells bearing receptors for both sheep erythrocytes (sE) and complement (C′), a combined rosette assay was developed using uncoated sE and chicken erythrocytes coated with antibody and complement (cEAC′). Cells with both receptors were present in low percentages in the blood of normal individuals. Tissues and/or blood from 543 patients with various diseases were studied using the combined rosette assay. Large numbers of malignant cells were found to carry receptors for both C′ and sE in four patients with lymphoproliferative malignancies. Two were children with lymphoma who had mediastinal masses. The other two were adults with chronic lymphocytic leukemia; their cells also carried surface immunoglobulin and Fc receptors. In these four patients double or multiple receptor lymphocytes may represent a clonal proliferation of subsets of lymphocytes present in small numbers under normal conditions. An increase in double receptor lymphocytes was rare in the non-neoplastic conditions studied and seen only in children. Double receptor lymphocytes may represent an immature or {"}stem{"} cell lymphocyte subset.",

author = "Gajl-Peczalska, {Kazimiera J.} and Sandra Chartrand and Bloomfield, {Clara D.} and Jean Corte and Coccia, {Peter F.} and Nesbit, {Mark E.} and Kersey, {John H.}",

note = "Funding Information: The excellent technical assistance of Mrs. Mary Buechele, Mrs. Tanya Ivanov, and Mrs. Jacqueline Farmer and the secretarial and editorial assistance of Ms. Susan Perry are acknowledged. This work was supported in part by grants from USPHS (CA-17034, CA-16228, CA-08832. CA-15548, CA-07306, CA-05158, CA-16450, CA-1 1996, and CA-08101). the Leukemia Task Force (No. 37). Masonic Memorial Hospital Fund, Inc.. and the Minnesota Medical Foundation.",

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AU - Gajl-Peczalska, Kazimiera J.

AU - Chartrand, Sandra

AU - Bloomfield, Clara D.

AU - Corte, Jean

AU - Coccia, Peter F.

AU - Nesbit, Mark E.

AU - Kersey, John H.

N1 - Funding Information: The excellent technical assistance of Mrs. Mary Buechele, Mrs. Tanya Ivanov, and Mrs. Jacqueline Farmer and the secretarial and editorial assistance of Ms. Susan Perry are acknowledged. This work was supported in part by grants from USPHS (CA-17034, CA-16228, CA-08832. CA-15548, CA-07306, CA-05158, CA-16450, CA-1 1996, and CA-08101). the Leukemia Task Force (No. 37). Masonic Memorial Hospital Fund, Inc.. and the Minnesota Medical Foundation.

PY - 1977/9

Y1 - 1977/9

N2 - To identify lymphoid cells bearing receptors for both sheep erythrocytes (sE) and complement (C′), a combined rosette assay was developed using uncoated sE and chicken erythrocytes coated with antibody and complement (cEAC′). Cells with both receptors were present in low percentages in the blood of normal individuals. Tissues and/or blood from 543 patients with various diseases were studied using the combined rosette assay. Large numbers of malignant cells were found to carry receptors for both C′ and sE in four patients with lymphoproliferative malignancies. Two were children with lymphoma who had mediastinal masses. The other two were adults with chronic lymphocytic leukemia; their cells also carried surface immunoglobulin and Fc receptors. In these four patients double or multiple receptor lymphocytes may represent a clonal proliferation of subsets of lymphocytes present in small numbers under normal conditions. An increase in double receptor lymphocytes was rare in the non-neoplastic conditions studied and seen only in children. Double receptor lymphocytes may represent an immature or "stem" cell lymphocyte subset.

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Lymphocytes bearing receptors for both sheep erythrocytes and complement in patients with neoplastic and non-neoplastic diseases

Abstract

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