TY - JOUR
T1 - Lysobacter enzymogenes employs diverse genes for inhibiting hypha growth and spore germination of soybean fungal pathogens
AU - Yu, Menghao
AU - Zhang, Guiying
AU - Jiang, Jiasong
AU - Du, Liangcheng
AU - Zhao, Youfu
PY - 2020
Y1 - 2020
N2 - Lysobacter enzymogenes strain C3 (LeC3) is a potential biocontrol agent for plant diseases caused by fungi and oomycetes. Understanding the interaction between LeC3 and soybean pathogens at the molecular level could help improve its biocontrol efficacy. In this study, we obtained mutants with decreased abilities in inhibiting hypha growth of the white mold pathogen Sclerotinia sclerotiorum. Insertion sites for 50 mutants, which no longer inhibited S. sclerotiorum hypha growth in dual cultural assay, were determined and seven mutants were selected for further characterization. These seven mutants also completely lost their abilities in suppressing spore germination of Fusarium virguliforme, the causal agent of soybean sudden death syndrome. Furthermore, mutation of the seven genes, which encode diguanylate cyclase, transcriptional regulators from the TetR family, hemolysin III family channel protein, type IV secretion system VirB10 protein, phenol hydroxylase, and phosphoadenosine phosphosulfate reductase, respectively, led to reduced production or secretion of four extracellular enzymes and heat-stable antifungal factor (HSAF). These results suggest that these seven genes play important roles in L. enzymogenes in suppressing hypha growth and spore germination of fungal pathogens, probably by influencing production or secretion of extracellular enzymes and HSAF.
AB - Lysobacter enzymogenes strain C3 (LeC3) is a potential biocontrol agent for plant diseases caused by fungi and oomycetes. Understanding the interaction between LeC3 and soybean pathogens at the molecular level could help improve its biocontrol efficacy. In this study, we obtained mutants with decreased abilities in inhibiting hypha growth of the white mold pathogen Sclerotinia sclerotiorum. Insertion sites for 50 mutants, which no longer inhibited S. sclerotiorum hypha growth in dual cultural assay, were determined and seven mutants were selected for further characterization. These seven mutants also completely lost their abilities in suppressing spore germination of Fusarium virguliforme, the causal agent of soybean sudden death syndrome. Furthermore, mutation of the seven genes, which encode diguanylate cyclase, transcriptional regulators from the TetR family, hemolysin III family channel protein, type IV secretion system VirB10 protein, phenol hydroxylase, and phosphoadenosine phosphosulfate reductase, respectively, led to reduced production or secretion of four extracellular enzymes and heat-stable antifungal factor (HSAF). These results suggest that these seven genes play important roles in L. enzymogenes in suppressing hypha growth and spore germination of fungal pathogens, probably by influencing production or secretion of extracellular enzymes and HSAF.
KW - Bacteriology
KW - Biocontrol
KW - C-di-GMP
KW - Genetics and resistance
KW - Molecular mechanism
KW - Type 4 secretion system
UR - http://www.scopus.com/inward/record.url?scp=85081075382&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85081075382&partnerID=8YFLogxK
U2 - 10.1094/PHYTO-09-19-0356-R
DO - 10.1094/PHYTO-09-19-0356-R
M3 - Article
C2 - 31774360
AN - SCOPUS:85081075382
VL - 110
SP - 593
EP - 602
JO - Phytopathology
JF - Phytopathology
SN - 0031-949X
IS - 3
ER -