TY - JOUR
T1 - Lytic viruses infecting a chlorella-like alga
AU - Van Etten, James L.
AU - Burbank, Dwight E.
AU - Schuster, Anne M.
AU - Meints, Russel H.
N1 - Funding Information:
We thank Richard Starr, Pete John, and Takashi Yamada for providing some of the Chloreua cultures and Cecil Van Etten for collecting the water samples from Illinois. Numerous discussions with Myron Brakke and Les Lane are gratefully acknowledged. This investigation was supported, in part, by Public Health Service Grant GM-32441 from the National Institute of General Medicine and Grant DE-ACOS-82ER12086 from the Department of Energy.
PY - 1985/1/15
Y1 - 1985/1/15
N2 - A number of viruses which form plaques on the unicellular, eukaryotic, Chlorella-like green alga, strain NC64A, were isolated from fresh water ponds and rivers in Illinois, North Carolina, and South Carolina. The viruses were large polyhedrons (160 to 190 nm in diameter) and contained dsDNA genomes of ca. 300 kbp. All of the viral DNAs hybridized with DNA from the previously described PBCV-1 virus. However, the viruses, even many of those isolated from the same water sample, could be distinguished from one another by DNA restriction endonuclease digestion. The viruses, including PBCV-1, were grouped into five classes by their resistance to certain DNA restriction endonucleases. Presumably the DNAs in the five classes contain different types or amounts of modified bases.
AB - A number of viruses which form plaques on the unicellular, eukaryotic, Chlorella-like green alga, strain NC64A, were isolated from fresh water ponds and rivers in Illinois, North Carolina, and South Carolina. The viruses were large polyhedrons (160 to 190 nm in diameter) and contained dsDNA genomes of ca. 300 kbp. All of the viral DNAs hybridized with DNA from the previously described PBCV-1 virus. However, the viruses, even many of those isolated from the same water sample, could be distinguished from one another by DNA restriction endonuclease digestion. The viruses, including PBCV-1, were grouped into five classes by their resistance to certain DNA restriction endonucleases. Presumably the DNAs in the five classes contain different types or amounts of modified bases.
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U2 - 10.1016/0042-6822(85)90452-0
DO - 10.1016/0042-6822(85)90452-0
M3 - Article
C2 - 2981448
AN - SCOPUS:0021991927
SN - 0042-6822
VL - 140
SP - 135
EP - 143
JO - Virology
JF - Virology
IS - 1
ER -