MAC5, an RNA-binding protein, protects pri-miRNAs from SERRATE-dependent exoribonuclease activities

Shengjun Li, Mu Li, Kan Liu, Huimin Zhang, Shuxin Zhang, Chi Zhang, Bin Yu

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


MAC5 is a component of the conservedMOS4-associated complex. It plays critical roles in development and immunity. Here we report that MAC5 is required for microRNA (miRNA) biogenesis. MAC5 interacts with Serrate (SE), which is a core component of the microprocessor that processes primary miRNA transcripts (pri-miRNAs) into miRNAs and binds the stem-loop region of pri-miRNAs. MAC5 is essential for both the efficient processing and the stability of primiRNAs. Interestingly, the reduction of pri-miRNA levels in mac5 is partially caused by XRN2/XRN3, the nuclear-localized 5'-to-3' exoribonucleases, and depends on SE. These results reveal that MAC5 plays a dual role in promoting pri-miRNA processing and stability through its interaction with SE and/or pri-miRNAs. This study also uncovers that pri-miRNAs need to be protected from nuclear RNA decay machinery, which is connected to the microprocessor.

Original languageEnglish (US)
Pages (from-to)23982-23990
Number of pages9
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number38
StatePublished - Sep 22 2020


  • MAC5A
  • MiRNA biogenesis
  • Pri-miRNA stablity
  • Serrate

ASJC Scopus subject areas

  • General


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