Magnetic resonance microscopy for monitoring osteogenesis in tissue-engineered construct in vitro

Huihui Xu, Shadi F. Othman, Liu Hong, Ioana A. Peptan, Richard L. Magin

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Magnetic resonance microscopy (MRM) is used to monitor osteogenesis in tissue-engineered constructs. Measurements of the developing tissue's MR relaxation times (T1 and T2), apparent diffusion coefficient (ADC) and elastic shear modulus were conducted over a 4-week growth period using an 11.74 T Bruker spectrometer with an imaging probe adapted for MR elastography (MRE). Both the relaxation times and the ADC show a statistically significant decrease after only one week of tissue development while the tissue stiffness increases progressively during the first two weeks of in vitro growth. The measured MR parameters are correlated with histologically monitored osteogenic tissue development. This study shows that MRM can provide quantitative data with which to characterize the growth and development of tissue-engineered bone.

Original languageEnglish (US)
Pages (from-to)719-732
Number of pages14
JournalPhysics in medicine and biology
Volume51
Issue number3
DOIs
StatePublished - Feb 7 2006

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

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