Maize pyruvate decarboxylase mRNA is induced anaerobically

Philip M. Kelley

doi:10.1007/BF00016139

Maize pyruvate decarboxylase mRNA is induced anaerobically

Philip M. Kelley

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

A cDNA was identified using an oligonucleotide designed by comparing the sequences of bacterial and yeast pyruvate decarboxylase. The sequence of the cDNA identified by the oligonucleotide contained an open reading frame that encoded a protein of 65 kDa that was similar in sequence to bacterial and yeast pyruvate decarboxylase. This protein was selectively precipitated by an antiserum specific for maize PDC. Northern-blot analysis shows that PDC mRNA is anaerobically induced. Southern-blot analysis of maize genomic DNA indicated that the maize PDC gene has a single or low copy number.

Original language	English (US)
Pages (from-to)	213-222
Number of pages	10
Journal	Plant Molecular Biology
Volume	13
Issue number	2
DOIs	https://doi.org/10.1007/BF00016139
State	Published - Aug 1989
Externally published	Yes

Keywords

anaerobic stress
gene regulation
maize
plant
pyruvate decarboxylase
sequence

ASJC Scopus subject areas

Genetics
Agronomy and Crop Science
Plant Science

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10.1007/BF00016139

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title = "Maize pyruvate decarboxylase mRNA is induced anaerobically",

abstract = "A cDNA was identified using an oligonucleotide designed by comparing the sequences of bacterial and yeast pyruvate decarboxylase. The sequence of the cDNA identified by the oligonucleotide contained an open reading frame that encoded a protein of 65 kDa that was similar in sequence to bacterial and yeast pyruvate decarboxylase. This protein was selectively precipitated by an antiserum specific for maize PDC. Northern-blot analysis shows that PDC mRNA is anaerobically induced. Southern-blot analysis of maize genomic DNA indicated that the maize PDC gene has a single or low copy number.",

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year = "1989",

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AU - Kelley, Philip M.

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AB - A cDNA was identified using an oligonucleotide designed by comparing the sequences of bacterial and yeast pyruvate decarboxylase. The sequence of the cDNA identified by the oligonucleotide contained an open reading frame that encoded a protein of 65 kDa that was similar in sequence to bacterial and yeast pyruvate decarboxylase. This protein was selectively precipitated by an antiserum specific for maize PDC. Northern-blot analysis shows that PDC mRNA is anaerobically induced. Southern-blot analysis of maize genomic DNA indicated that the maize PDC gene has a single or low copy number.

KW - anaerobic stress

KW - gene regulation

KW - maize

KW - plant

KW - pyruvate decarboxylase

KW - sequence

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JO - Plant Molecular Biology

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IS - 2

ER -

Maize pyruvate decarboxylase mRNA is induced anaerobically

Abstract

Keywords

ASJC Scopus subject areas

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