Malonate: A versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals

Todd Holyoak; Timothy D. Fenn; Mark A. Wilson; Aaron G. Moulin; Dagmar Ringe; Gregory A. Petsko

doi:10.1107/S0907444903021784

Malonate: A versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals

Todd Holyoak, Timothy D. Fenn, Mark A. Wilson, Aaron G. Moulin, Dagmar Ringe, Gregory A. Petsko

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Collection of quality data on high-energy synchrotron sources requires the selection of a cryoprotectant that allows protein crystals to be cryocooled without damage to the crystal and with suppression of ice formation. The use of sodium malonate as a versatile cryoprotectant for salt-grown protein crystals is presented here. In addition to its useful cryoprotectant properties, sodium malonate can also function as a versatile stabilizing solution that allows the manipulation, derivatization and ligand soaking of crystals grown from salt that may not be possible in the crystal mother liquor.

Original language	English (US)
Pages (from-to)	2356-2358
Number of pages	3
Journal	Acta Crystallographica - Section D Biological Crystallography
Volume	59
Issue number	12
DOIs	https://doi.org/10.1107/S0907444903021784
State	Published - Dec 2003
Externally published	Yes

ASJC Scopus subject areas

Structural Biology

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abstract = "Collection of quality data on high-energy synchrotron sources requires the selection of a cryoprotectant that allows protein crystals to be cryocooled without damage to the crystal and with suppression of ice formation. The use of sodium malonate as a versatile cryoprotectant for salt-grown protein crystals is presented here. In addition to its useful cryoprotectant properties, sodium malonate can also function as a versatile stabilizing solution that allows the manipulation, derivatization and ligand soaking of crystals grown from salt that may not be possible in the crystal mother liquor.",

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AU - Holyoak, Todd

AU - Fenn, Timothy D.

AU - Wilson, Mark A.

AU - Moulin, Aaron G.

AU - Ringe, Dagmar

AU - Petsko, Gregory A.

PY - 2003/12

Y1 - 2003/12

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AB - Collection of quality data on high-energy synchrotron sources requires the selection of a cryoprotectant that allows protein crystals to be cryocooled without damage to the crystal and with suppression of ice formation. The use of sodium malonate as a versatile cryoprotectant for salt-grown protein crystals is presented here. In addition to its useful cryoprotectant properties, sodium malonate can also function as a versatile stabilizing solution that allows the manipulation, derivatization and ligand soaking of crystals grown from salt that may not be possible in the crystal mother liquor.

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Malonate: A versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals

Abstract

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