Collection of quality data on high-energy synchrotron sources requires the selection of a cryoprotectant that allows protein crystals to be cryocooled without damage to the crystal and with suppression of ice formation. The use of sodium malonate as a versatile cryoprotectant for salt-grown protein crystals is presented here. In addition to its useful cryoprotectant properties, sodium malonate can also function as a versatile stabilizing solution that allows the manipulation, derivatization and ligand soaking of crystals grown from salt that may not be possible in the crystal mother liquor.
|Original language||English (US)|
|Number of pages||3|
|Journal||Acta Crystallographica - Section D Biological Crystallography|
|State||Published - Dec 2003|
ASJC Scopus subject areas
- Structural Biology