TY - JOUR
T1 - Maternal opioid use disorder
T2 - Placental transcriptome analysis for neonatal opioid withdrawal syndrome
AU - Radhakrishna, Uppala
AU - Nath, Swapan K.
AU - Vishweswaraiah, Sangeetha
AU - Uppala, Lavanya V.
AU - Forray, Ariadna
AU - Muvvala, Srinivas B.
AU - Mishra, Nitish K.
AU - Southekal, Siddesh
AU - Guda, Chittibabu
AU - Govindamangalam, Hiranjith
AU - Vargas, Derek
AU - Gardella, William G.
AU - Crist, Richard C.
AU - Berrettini, Wade H.
AU - Metpally, Raghu P.
AU - Bahado-Singh, Ray O.
N1 - Publisher Copyright:
© 2021
PY - 2021/11
Y1 - 2021/11
N2 - Excessive prenatal opioid exposure may lead to the development of Neonatal Opioid Withdrawal Syndrome (NOWS). RNA-seq was done on 64 formalin-fixed paraffin-embedded placental tissue samples from 32 mothers with opioid use disorder, with newborns with NOWS that required treatment, and 32 prenatally unexposed controls. We identified 93 differentially expressed genes in the placentas of infants with NOWS compared to unexposed controls. There were 4 up- and 89 downregulated genes. Among these, 7 genes CYP1A1, APOB, RPH3A, NRXN1, LINC01206, AL157396.1, UNC80 achieved an FDR p-value of <0.01. The remaining 87 genes were significant with FDR p-value <0.05. The 4 upregulated, CYP1A1, FP671120.3, RAD1, RN7SL856P, and the 10 most significantly downregulated genes were RNA5SP364, GRIN2A, UNC5D, DMBT1P1, MIR3976HG, LINC02199, LINC02822, PANTR1, AC012178.1, CTNNA2. Ingenuity Pathway Analysis identified the 7 most likely to play an important role in the etiology of NOWS. Our study expands insights into the genetic mechanisms of NOWS development.
AB - Excessive prenatal opioid exposure may lead to the development of Neonatal Opioid Withdrawal Syndrome (NOWS). RNA-seq was done on 64 formalin-fixed paraffin-embedded placental tissue samples from 32 mothers with opioid use disorder, with newborns with NOWS that required treatment, and 32 prenatally unexposed controls. We identified 93 differentially expressed genes in the placentas of infants with NOWS compared to unexposed controls. There were 4 up- and 89 downregulated genes. Among these, 7 genes CYP1A1, APOB, RPH3A, NRXN1, LINC01206, AL157396.1, UNC80 achieved an FDR p-value of <0.01. The remaining 87 genes were significant with FDR p-value <0.05. The 4 upregulated, CYP1A1, FP671120.3, RAD1, RN7SL856P, and the 10 most significantly downregulated genes were RNA5SP364, GRIN2A, UNC5D, DMBT1P1, MIR3976HG, LINC02199, LINC02822, PANTR1, AC012178.1, CTNNA2. Ingenuity Pathway Analysis identified the 7 most likely to play an important role in the etiology of NOWS. Our study expands insights into the genetic mechanisms of NOWS development.
KW - Differentially expressed genes
KW - In utero drug exposure
KW - Neonatal abstinence syndrome
KW - Transcription factors
KW - Transcriptional regulatory element
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U2 - 10.1016/j.ygeno.2021.08.001
DO - 10.1016/j.ygeno.2021.08.001
M3 - Article
C2 - 34352367
AN - SCOPUS:85122548872
SN - 0888-7543
VL - 113
SP - 3610
EP - 3617
JO - Genomics
JF - Genomics
IS - 6
ER -