MBP-1 upregulates miR-29b, which represses Mcl-1, collagens, and matrix metalloproteinase-2 in prostate cancer cells

Robert Steele, Justin L. Mott, Ratna B. Ray

Research output: Contribution to journalReview articlepeer-review

103 Scopus citations

Abstract

c-myc promoter binding protein (MBP-1) is a multifunctional protein known to regulate expression of targets involved in the malignant phenotype. We have previously demonstrated that exogenous expression of MBP-1 inhibits prostate tumor growth, although the mechanism of growth inhibition is not well understood. We hypothesized that MBP-1 may modulate microRNA (miRNA) expression for regulation of prostate cancer cell growth. In this study, we demonstrated that exogenous MBP-1 upregulates miR-29b by 5- to 9-fold in prostate cancer cells as measured by real-time quantitative reverse transcription polymerase chain reaction. Subsequent studies indicated that exogenous expression of miR-29b inhibited Mcl-1, COL1A1, and COL4A1. Furthermore, a novel target with potential implications for invasion and metastasis, matrix metallopeptidase-2 (MMP-2), was identified and confirmed to be a miR-29b target in prostate cancer cells. Together, these results demonstrated that exogenous expression of miR-29b regulates prostate cancer cell growth by modulating antiapoptotic and prometastatic matrix molecules, implicating the therapeutic potential of miR-29b for prostate cancer inhibition.

Original languageEnglish (US)
Pages (from-to)381-387
Number of pages7
JournalGenes and Cancer
Volume1
Issue number4
DOIs
StatePublished - 2010
Externally publishedYes

Keywords

  • Extracellular matrix molecules
  • Mcl-1
  • MicroRNA
  • Prostate cancer

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

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