Measurement of plasma uracil using gas chromatography-mass spectrometry in normal individuals and in patients receiving inhibitors of dihydropyrimidine dehydrogenase

Daoqin Bi; Lawrence W. Anderson; Jeremy Shapiro; Alla Shapiro; Jean L. Grem; Chris H. Takimoto

doi:10.1016/S0378-4347(99)00528-9

Measurement of plasma uracil using gas chromatography-mass spectrometry in normal individuals and in patients receiving inhibitors of dihydropyrimidine dehydrogenase

Daoqin Bi, Lawrence W. Anderson, Jeremy Shapiro, Alla Shapiro, Jean L. Grem, Chris H. Takimoto

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

A sensitive gas chromatographic-mass spectrometric method is described for reliably measuring endogenous uracil in 100 μl of human plasma. Validation of this assay over a wide concentration range, 0.025 ♂m to 250 ♂m (0.0028 μg/ml to 28 μg/ml), allowed for the determination of plasma uracil in patients treated with agents such as eniluracil, an inhibitor of the pyrimidine catabolic enzyme, dihydropyrimidine dehydrogenase. Calibration standards were prepared in human plasma using the stable isotope, [¹⁵N₂]uracil, to avoid interference from endogenous uracil and 10 ♂m 5- chlorouracil was added as the internal standard.

Original language	English (US)
Pages (from-to)	249-258
Number of pages	10
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	738
Issue number	2
DOIs	https://doi.org/10.1016/S0378-4347(99)00528-9
State	Published - Feb 11 2000
Externally published	Yes

Keywords

Dihydropyrimidine dehydrogenase
Eniluracil
Uracil

ASJC Scopus subject areas

General Chemistry

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10.1016/S0378-4347(99)00528-9

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Bi, D., Anderson, L. W., Shapiro, J., Shapiro, A., Grem, J. L., & Takimoto, C. H. (2000). Measurement of plasma uracil using gas chromatography-mass spectrometry in normal individuals and in patients receiving inhibitors of dihydropyrimidine dehydrogenase. Journal of Chromatography B: Biomedical Sciences and Applications, 738(2), 249-258. https://doi.org/10.1016/S0378-4347(99)00528-9

Measurement of plasma uracil using gas chromatography-mass spectrometry in normal individuals and in patients receiving inhibitors of dihydropyrimidine dehydrogenase. / Bi, Daoqin; Anderson, Lawrence W.; Shapiro, Jeremy et al.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 738, No. 2, 11.02.2000, p. 249-258.

Research output: Contribution to journal › Article › peer-review

Bi, D, Anderson, LW, Shapiro, J, Shapiro, A, Grem, JL & Takimoto, CH 2000, 'Measurement of plasma uracil using gas chromatography-mass spectrometry in normal individuals and in patients receiving inhibitors of dihydropyrimidine dehydrogenase', Journal of Chromatography B: Biomedical Sciences and Applications, vol. 738, no. 2, pp. 249-258. https://doi.org/10.1016/S0378-4347(99)00528-9

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AU - Grem, Jean L.

AU - Takimoto, Chris H.

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Measurement of plasma uracil using gas chromatography-mass spectrometry in normal individuals and in patients receiving inhibitors of dihydropyrimidine dehydrogenase

Abstract

Keywords

ASJC Scopus subject areas

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