Abstract
We investigated the utility of [3H]puromycin as an alternate and adjunct precursor to amino acids for measuring protein synthetic activity in rat liver slices. Slices were incubated in the presence of either [3H]puromycin or radiolabeled valine to compare the incorporation of these isotopic precursors into nascent hepatocellular proteins. Compared to liver slices from controls, comparable decreases in the incorporation of both [3H]puromycin and labeled valine were observed in experiments using slices from fasted rats and in slices preincubated with 25 mw ethanol. Radiolabeling of nascent polypeptides with either [3H]puromycin or labeled valine in liver slices from rats fed a liquid diet containing ethanol was also decreased compared to slices from pair‐fed control and chow‐fed animals. Our results demonstrated the validity of using [3H]puromycin to detect changes in protein synthetic activity under these conditions. The potential advantage of using [3H]puromycin for in vivo studies is discussed.
Original language | English (US) |
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Pages (from-to) | 526-530 |
Number of pages | 5 |
Journal | Alcoholism: Clinical and Experimental Research |
Volume | 9 |
Issue number | 6 |
DOIs | |
State | Published - Nov 1985 |
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Toxicology
- Psychiatry and Mental health