@inbook{483ad842afe8465bba47998f888296ed,
title = "Measuring binding constants of his-tagged proteins using affinity chromatography and Ni-NTA immobilized enzymes",
abstract = "Affinity chromatography is one way to measure the binding constants of a protein–ligand interaction. Here, we describe a method of measuring a binding constant using Ni-NTA resin to immobilize a His-tagged enzyme and the method of frontal analysis. While other methods of immobilization are possible, using the strong affinity interaction between His-tagged proteins and Ni-NTA supports results in a fast, easy, and gentle method of immobilization. Once the affinity support is created, frontal analysis can be used to measure the binding constant between the protein and various analytes.",
keywords = "Affinity chromatography, Binding constant, Enzyme immobilization, Frontal analysis",
author = "Moser, {Annette C.} and Benjamin White and Kovacs, {Frank A.}",
note = "Funding Information: This work was supported by the UNK Summer Student Research Program, the UNK Undergraduate Research Fellows Program, and the UNK Chemistry Department. Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2021.",
year = "2021",
doi = "10.1007/978-1-0716-0775-6_26",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "405--416",
booktitle = "Methods in Molecular Biology",
}