TY - JOUR
T1 - Mechanism of c-Myb-C/EBPβ cooperation from separated sites on a promoter
AU - Tahirov, Tahir H.
AU - Sato, Ko
AU - Ichikawa-Iwata, Emi
AU - Sasaki, Motoko
AU - Inoue-Bungo, Taiko
AU - Shiina, Masaaki
AU - Kimura, Kazumi
AU - Takata, Shioka
AU - Fujikawa, Atsushi
AU - Morii, Hisayuki
AU - Kumasaka, Takashi
AU - Yamamoto, Masaki
AU - Ishii, Shunsuke
AU - Ogata, Kazuhiro
PY - 2002/1/11
Y1 - 2002/1/11
N2 - c-Myb, but not avian myeloblastosis virus (AMV) v-Myb, cooperates with C/EBPβ to regulate transcription of myeloid-specific genes. To assess the structural basis for that difference, we determined the crystal structures of complexes comprised of the c-Myb or AMV v-Myb DNA-binding domain (DBD), the C/EBPβ DBD, and a promoter DNA fragment. Within the c-Myb complex, a DNA-bound C/EBPβ interacts with R2 of c-Myb bound to a different DNA fragment; point mutations in v-Myb R2 eliminate such interaction within the v-Myb complex. GST pull-down assays, luciferase trans-activation assays, and atomic force microscopy confirmed that the interaction of c-Myb and C/EBPβ observed in crystal mimics their long range interaction on the promoter, which is accompanied by intervening DNA looping.
AB - c-Myb, but not avian myeloblastosis virus (AMV) v-Myb, cooperates with C/EBPβ to regulate transcription of myeloid-specific genes. To assess the structural basis for that difference, we determined the crystal structures of complexes comprised of the c-Myb or AMV v-Myb DNA-binding domain (DBD), the C/EBPβ DBD, and a promoter DNA fragment. Within the c-Myb complex, a DNA-bound C/EBPβ interacts with R2 of c-Myb bound to a different DNA fragment; point mutations in v-Myb R2 eliminate such interaction within the v-Myb complex. GST pull-down assays, luciferase trans-activation assays, and atomic force microscopy confirmed that the interaction of c-Myb and C/EBPβ observed in crystal mimics their long range interaction on the promoter, which is accompanied by intervening DNA looping.
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U2 - 10.1016/S0092-8674(01)00636-5
DO - 10.1016/S0092-8674(01)00636-5
M3 - Article
C2 - 11792321
AN - SCOPUS:18244394860
VL - 108
SP - 57
EP - 70
JO - Cell
JF - Cell
SN - 0092-8674
IS - 1
ER -