Abstract

33B rat Schwannoma cell line is known to exhibit phagocytic properties analogous to those of normal Schwann cells. The mechanism of phagocytosis by this cell line was investigated by studying the effect of known modulators of phagocytosis on the uptake of latex particles by these cells. Treatments which block energy production of the host cell, such as incubation at 4°C and treatment with sodium azide, markedly inhibited the phagocytosis of latex particles by these cells. Phagocytosis was dose-dependently, and completely, inhibited by cytochalasin B, demonstrating an important role of microfilaments. Colchicine produced a minor inhibition of phagocytosis only at the highest concentration (10-3 M) tested, suggesting that intact microtubules are not crucial for latex phagocytosis. Dibutyryl cyclic AMP was without any effect on the phagocytosis. Thus, latex phagocytosis by rat Schwannoma cells is an active, energy-dependent process requiring intact microfilaments with only a minor dependence on microtubules.

Original languageEnglish (US)
Pages (from-to)113-119
Number of pages7
JournalJournal of the neurological sciences
Volume75
Issue number1
DOIs
StatePublished - Aug 1986

Keywords

  • Colchicine
  • Cyclic AMP
  • Cytochalasin
  • Microfilaments
  • Microtubules
  • Phagocytosis
  • Schwann cell

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

Fingerprint Dive into the research topics of 'Mechanism of phagocytosis by Schwann cells'. Together they form a unique fingerprint.

  • Cite this