Melanocytes do not migrate directionally in physiological DC electric fields

Jennifer C. Grahn, Debra A. Reilly, Richard L. Nuccitelli, Rivkah R. Isseroff

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Wounding skin generates an endogenous electric field of 100-200 mV/mm in the immediate vicinity of the wound. When keratinocytes are exposed to direct current electric fields of this magnitude, they exhibit galvanotaxis, or directional migration toward the cathode, suggesting that wound-generated electric fields provide migrational cues that contribute to wound healing. Because melanocytes must also migrate into the healing wound to repigment it, their motility in response to electric fields of physiologic magnitude was examined. Human skin-derived melanocytes, either exposed to 100 mV/mm direct current electric fields or nonexposed controls, both exhibited motility rates of 9 μm/hour, significantly (three- to five-fold) lower than the motility rates of keratinocytes under identical conditions. However, in sharp contrast to keratinocytes, melanocytes exhibited no directional migration in the electric field. Additionally, neither the number of primary dendrites per cell, nor the orientation of the dendrites with respect to the field vector, nor the average length of the dendrites was significantly different in melanocytes exposed to the electric field as compared to nonexposed controls. Thus, in marked contrast to keratinocytes, human skin-derived melanocytes do not respond to direct current electric fields of physiologic magnitude with either directional migration or reorientation of dendrites. This may account for the delay in repigmentation that often accompanies wound reepithelialization.

Original languageEnglish (US)
Pages (from-to)64-70
Number of pages7
JournalWound Repair and Regeneration
Volume11
Issue number1
DOIs
StatePublished - Jan 2003

ASJC Scopus subject areas

  • Surgery
  • Dermatology

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