Metabolism of arylhydrazines by cytochrome P-450 mixed function oxidases and prostaglandin(H)synthase from mouse lungs

Terence Lawson

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The arylhydrazines 4-methylphenylhydrazine hydrochloride, N′-acetyl-4-methylphenylhydrazine and N′-acetyl-4-hydroxymethylphenylhydrazine (HMPH) were metabolized by ram seminal vesicle prostaglandin(H)synthase (P(H)S) and by cytochrome P-450- and P(H)S-dependent enzymes from mouse lung. Based on the Km-values, the cytochrome P-450 enzymes were the most efficient, suggesting that they would be responsible for the metabolic activation of these compounds in vivo. Cytochrome P-450-dependent metabolism was inhibited by metyrapone and SKF-525A, ruling out the involvement of flavin mono-oxygenase. Agaritine, an arylhydrazide, was poorly metabolized by both systems.

Original languageEnglish (US)
Pages (from-to)193-200
Number of pages8
JournalCancer Letters
Volume34
Issue number2
DOIs
StatePublished - Feb 1987

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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