Metabolomics analyses from tissues in parkinson’s disease

Fatema Bhinderwala, Shulei Lei, Jade Woods, Jordan Rose, Darrell D. Marshall, Eli Riekeberg, Aline De Lima Leite, Martha Morton, Eric D. Dodds, Rodrigo Franco, Robert Powers

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Metabolomics has been successfully applied to study neurological and neurodegenerative disorders including Parkinson’s disease for (1) the identification of potential biomarkers of onset and disease progression; (2) the identification of novel mechanisms of disease progression; and (3) the assessment of treatment prognosis and outcome. Reproducible and efficient extraction of metabolites is imperative to the success of any metabolomics investigation. Unlike other omics techniques, the composition of the metabolome can be negatively impacted by the preparation, processing, and handling of these samples. The proper choice of data collection, preprocessing, and processing protocols is similarly important to the design of an effective metabolomics experiment. Likewise, the correct application of univariate and multivariate statistical methods is essential for providing biologically relevant insights. In this chapter, we have outlined a detailed metabolomics workflow that addresses all of these issues. A step-by-step protocol from the preparation of neuronal cells and metabolomic tissue samples to their metabolic analyses using nuclear magnetic resonance, mass spectrometry, and chemometrics is presented.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages217-257
Number of pages41
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1996
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Chemometrics
  • Mass spectrometry
  • Metabolomics
  • NMR
  • Neurodegeneration
  • Parkinson’s disease

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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