Methamidophos, dichlorvos, O-methoate and diazinon pesticides used in Turkey make a covalent bond with butyrylcholinesterase detected by mass spectrometry

Ozden Tacal, Oksana Lockridge

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Organophosphorus pesticides used most commonly in Turkey include methamidophos, dichlorvos, O-methoate and diazinon. These toxic chemicals or their metabolites make a covalent bond with the active site serine of butyrylcholinesterase. Our goal was to identify the adducts that result from the reaction of human butyrylcholinesterase with these pesticides. Highly purified human butyrylcholinesterase was treated with a 20-fold molar excess of pesticide.The protein was denatured by boiling and digested with trypsin. MS and MSMS spectra of HPLC-purified peptides were acquired on a MALDI-TOF-TOF 4800 mass spectrometer. It was found that methamidophos added a mass of +93, consistent with addition of methoxy aminophosphate. A minor amount of adductwith an added mass of +109was also found. Dichlorvos and O-methoate both made dimethoxyphosphate (+108) and monomethoxyphosphate adducts (+94). Diazinon gave a novel adduct with an added mass of +152 consistent with diethoxythiophosphate. Inhibition of enzyme activity in the presence of diazinon developed slowly (15 h), concomitant with isomerization of diazinon via a thiono-thiolo rearrangement. The isomer of diazinon yielded diethoxyphosphate and monoethoxyphosphate adducts with added masses of +136 and +108. MSMS spectra confirmed that each of the pesticides studied made a covalent bond with serine 198 of butyrylcholinesterase. These results can be used to identify the class of pesticides to which a patient was exposed.

Original languageEnglish (US)
Pages (from-to)469-475
Number of pages7
JournalJournal of Applied Toxicology
Volume30
Issue number5
DOIs
StatePublished - Jul 2010

Keywords

  • Diazinon
  • Dichlorvos
  • Human butyrylcholinesterase
  • Mass spectrometry
  • Methamidophos
  • O-methoate
  • Organophosphorus pesticides
  • Thiono-thiolo

ASJC Scopus subject areas

  • Toxicology

Fingerprint Dive into the research topics of 'Methamidophos, dichlorvos, O-methoate and diazinon pesticides used in Turkey make a covalent bond with butyrylcholinesterase detected by mass spectrometry'. Together they form a unique fingerprint.

  • Cite this