Methods for isolation and identification of nanoparticle-containing subcellular compartments

Ari Nowacek; Irena Kadiu; Joellyn McMillan; Howard E. Gendelman

doi:10.1007/978-1-62703-336-7_6

Methods for isolation and identification of nanoparticle-containing subcellular compartments

Ari Nowacek, Irena Kadiu, Joellyn McMillan, Howard E. Gendelman

Research output: Chapter in Book/Report/Conference proceeding › Chapter

7 Scopus citations

Abstract

Nanoparticle-based drug delivery systems have considerable potential for improvement of drug stability, bioavailability, and reduced dosing frequency. Important technological advantages of nanoparticles include high carrier capacity across biological membranes and controlled drug release. Ultimately, success of nanodelivery systems depends on toxicologic issues associated with the understanding of the fate of nanocarriers and their polymeric constituents within the targeted cells. Here we describe a method for determining subcellular distribution of nanoparticles by isolation and identification of organelles that come in direct contact with these structures.

Original language	English (US)
Title of host publication	Cellular and Subcellular Nanotechnology
Subtitle of host publication	Methods and Protocols
Editors	Volkmar Weissig, Mark Olsen, Tamer Elbayoumi
Pages	47-55
Number of pages	9
DOIs	https://doi.org/10.1007/978-1-62703-336-7_6
State	Published - 2013

Publication series

Name	Methods in Molecular Biology
Volume	991
ISSN (Print)	1064-3745

Keywords

Drug delivery
Endosome
Macrophage
Nanoparticle
Subcellular trafficking

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-62703-336-7_6

Cite this

Methods for isolation and identification of nanoparticle-containing subcellular compartments. / Nowacek, Ari; Kadiu, Irena; McMillan, Joellyn et al.
Cellular and Subcellular Nanotechnology: Methods and Protocols. ed. / Volkmar Weissig; Mark Olsen; Tamer Elbayoumi. 2013. p. 47-55 (Methods in Molecular Biology; Vol. 991).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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AB - Nanoparticle-based drug delivery systems have considerable potential for improvement of drug stability, bioavailability, and reduced dosing frequency. Important technological advantages of nanoparticles include high carrier capacity across biological membranes and controlled drug release. Ultimately, success of nanodelivery systems depends on toxicologic issues associated with the understanding of the fate of nanocarriers and their polymeric constituents within the targeted cells. Here we describe a method for determining subcellular distribution of nanoparticles by isolation and identification of organelles that come in direct contact with these structures.

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Methods for isolation and identification of nanoparticle-containing subcellular compartments

Abstract

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Keywords

ASJC Scopus subject areas

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