TY - JOUR
T1 - Methods to detect apoptotic-like cell death in filamentous fungi.
AU - Semighini, Camile P.
AU - Harris, Steven D.
PY - 2010
Y1 - 2010
N2 - Fungi are capable of undergoing apoptotic-like cell death, and display many of the characteristic features of apoptosis observed in multicellular organisms. These features include nuclear condensation, DNA fragmentation, translocation of phosphatidylserine from the cytoplasmic to the extracellular side of the plasma membrane, and increased levels of reactive oxygen species (ROS). Several assays can be used to detect apoptotic cells, and here we describe adaptations of assays such as TUNEL, Annexin V, and Evan's Blue for the investigation of apoptotic-like cell death in fungal hyphae. We also present approaches for monitoring nuclear condensation and production of ROS.
AB - Fungi are capable of undergoing apoptotic-like cell death, and display many of the characteristic features of apoptosis observed in multicellular organisms. These features include nuclear condensation, DNA fragmentation, translocation of phosphatidylserine from the cytoplasmic to the extracellular side of the plasma membrane, and increased levels of reactive oxygen species (ROS). Several assays can be used to detect apoptotic cells, and here we describe adaptations of assays such as TUNEL, Annexin V, and Evan's Blue for the investigation of apoptotic-like cell death in fungal hyphae. We also present approaches for monitoring nuclear condensation and production of ROS.
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U2 - 10.1007/978-1-60761-611-5_20
DO - 10.1007/978-1-60761-611-5_20
M3 - Article
C2 - 20238276
AN - SCOPUS:77953643335
SN - 1064-3745
VL - 638
SP - 269
EP - 279
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -