Methods to generate a sequence-defined transposon mutant library in Staphylococcus epidermidis strain 1457

Todd J. Widhelm, Vijay Kumar Yajjala, Jennifer L. Endres, Paul D. Fey, Kenneth W. Bayles

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

Transposon mutant libraries are valuable resources to investigators studying bacterial species, including Staphylococcus epidermidis, which are difficult to genetically manipulate. Although sequence-defined transposon mutant libraries have been constructed in Staphylococcus aureus, no such library exists for S. epidermidis. Nevertheless, the study of Tn917-mediated mutations has been paramount in discovering unique aspects of S. epidermidis biology including initial adherence and accumulation during biofilm formation. Herein, we describe modifications to the methodology first described by Bae et al. to utilize the mariner-based transposon bursa aurealis to generate mutants in S. epidermidis strain 1457.

Original languageEnglish (US)
Title of host publicationStaphylococcus Epidermidis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages135-142
Number of pages8
ISBN (Print)9781627037358
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1106
ISSN (Print)1064-3745

Keywords

  • Bursa aurealis
  • Staphylococcus epidermidis
  • Transposon

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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