Methylation as a crucial step in plant microRNA biogenesis

Bin Yu, Zhiyong Yang, Junjie Li, Svetlana Minakhina, Maocheng Yang, Richard W. Padgett, Ruth Steward, Xuemei Chen

Research output: Contribution to journalArticle

658 Scopus citations

Abstract

Methylation on the base or the ribose is prevalent in eukaryotic ribosomal RNAs (rRNAs) and is thought to be crucial for ribosome biogenesis and function. Artificially introduced 2′-O-methyl groups in small interfering RNAs (siRNAs) can stabilize siRNAs in serum without affecting their activities in RNA interference in mammalian cells. Here, we show that plant microRNAs (miRNAs) have a naturally occurring methyl group on the ribose of the last nucleotide. Whereas methylation of rRNAs depends on guide RNAs, the methyltransferase protein HEN1 is sufficient to methylate miRNA/miRNA* duplexes. Our studies uncover a new and crucial step in plant miRNA biogenesis and have profound implications in the function of miRNAs.

Original languageEnglish (US)
Pages (from-to)932-935
Number of pages4
JournalScience
Volume307
Issue number5711
DOIs
StatePublished - Feb 11 2005

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Methylation as a crucial step in plant microRNA biogenesis'. Together they form a unique fingerprint.

  • Cite this

    Yu, B., Yang, Z., Li, J., Minakhina, S., Yang, M., Padgett, R. W., Steward, R., & Chen, X. (2005). Methylation as a crucial step in plant microRNA biogenesis. Science, 307(5711), 932-935. https://doi.org/10.1126/science.1107130