Micro fixture enabled in-situ imaging and manipulation of cell membrane protein

Carmen Kar Man Fung, Ruiguo Yang, King Wai Chiu Lai, Ning Xi, Kristina Seiffert-Sinha, Animesh A. Sinha

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper presents a new and reliable approach for in-situ imaging and manipulation of membrane protein molecules. The major problematic issue in medicine is the lack of fundamental understanding of the molecular mechanisms that cause major life-threatening and incurable diseases. The development of atomic force microscopy (AFM) opens an entirely new way to address these medical questions. A biocompatible porous polymer micro-mesh is designed for immobilizing living cells and protein molecules during AFM scanning. We demonstrate its usefulness by imaging cancerous B-cells as well as cell-junction molecules associated with living epithelial cells at high resolution. This technology can not only be used for imaging, but also for nanomanipulation. Current results suggest that this AFM nanomanipulation system and the novel cell fixture apparatus can be used to study the dynamic changes and function of cellular structures with unparalleled resolution both under physiological and pathophysiological conditions.

Original languageEnglish (US)
Title of host publication2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
Pages165-168
Number of pages4
DOIs
StatePublished - Nov 29 2010
Event5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010 - Xiamen, China
Duration: Jan 20 2010Jan 23 2010

Publication series

Name2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010

Other

Other5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
CountryChina
CityXiamen
Period1/20/101/23/10

Keywords

  • AFM
  • Cancer cell
  • Cell fixture
  • Cell junction

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Fung, C. K. M., Yang, R., Lai, K. W. C., Xi, N., Seiffert-Sinha, K., & Sinha, A. A. (2010). Micro fixture enabled in-situ imaging and manipulation of cell membrane protein. In 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010 (pp. 165-168). [5592178] (2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010). https://doi.org/10.1109/NEMS.2010.5592178