Micro fixture enabled in-situ imaging and manipulation of cell membrane protein

Carmen Kar Man Fung; Ruiguo Yang; King Wai Chiu Lai; Ning Xi; Kristina Seiffert-Sinha; Animesh A. Sinha

doi:10.1109/NEMS.2010.5592178

Micro fixture enabled in-situ imaging and manipulation of cell membrane protein

Carmen Kar Man Fung, Ruiguo Yang, King Wai Chiu Lai, Ning Xi, Kristina Seiffert-Sinha, Animesh A. Sinha

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This paper presents a new and reliable approach for in-situ imaging and manipulation of membrane protein molecules. The major problematic issue in medicine is the lack of fundamental understanding of the molecular mechanisms that cause major life-threatening and incurable diseases. The development of atomic force microscopy (AFM) opens an entirely new way to address these medical questions. A biocompatible porous polymer micro-mesh is designed for immobilizing living cells and protein molecules during AFM scanning. We demonstrate its usefulness by imaging cancerous B-cells as well as cell-junction molecules associated with living epithelial cells at high resolution. This technology can not only be used for imaging, but also for nanomanipulation. Current results suggest that this AFM nanomanipulation system and the novel cell fixture apparatus can be used to study the dynamic changes and function of cellular structures with unparalleled resolution both under physiological and pathophysiological conditions.

Original language	English (US)
Title of host publication	2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
Pages	165-168
Number of pages	4
DOIs	https://doi.org/10.1109/NEMS.2010.5592178
State	Published - 2010
Externally published	Yes
Event	5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010 - Xiamen, China Duration: Jan 20 2010 → Jan 23 2010

Publication series

Name	2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010

Other

Other	5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
Country/Territory	China
City	Xiamen
Period	1/20/10 → 1/23/10

Keywords

AFM
Cancer cell
Cell fixture
Cell junction

ASJC Scopus subject areas

Control and Systems Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/NEMS.2010.5592178

Cite this

Fung, C. K. M., Yang, R., Lai, K. W. C., Xi, N., Seiffert-Sinha, K., & Sinha, A. A. (2010). Micro fixture enabled in-situ imaging and manipulation of cell membrane protein. In 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010 (pp. 165-168). Article 5592178 (2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010). https://doi.org/10.1109/NEMS.2010.5592178

Micro fixture enabled in-situ imaging and manipulation of cell membrane protein. / Fung, Carmen Kar Man; Yang, Ruiguo; Lai, King Wai Chiu et al.
2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010. 2010. p. 165-168 5592178 (2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Fung, CKM, Yang, R, Lai, KWC, Xi, N, Seiffert-Sinha, K & Sinha, AA 2010, Micro fixture enabled in-situ imaging and manipulation of cell membrane protein. in 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010., 5592178, 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010, pp. 165-168, 5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010, Xiamen, China, 1/20/10. https://doi.org/10.1109/NEMS.2010.5592178

Fung CKM, Yang R, Lai KWC, Xi N, Seiffert-Sinha K, Sinha AA. Micro fixture enabled in-situ imaging and manipulation of cell membrane protein. In 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010. 2010. p. 165-168. 5592178. (2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010). doi: 10.1109/NEMS.2010.5592178

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AU - Sinha, Animesh A.

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AB - This paper presents a new and reliable approach for in-situ imaging and manipulation of membrane protein molecules. The major problematic issue in medicine is the lack of fundamental understanding of the molecular mechanisms that cause major life-threatening and incurable diseases. The development of atomic force microscopy (AFM) opens an entirely new way to address these medical questions. A biocompatible porous polymer micro-mesh is designed for immobilizing living cells and protein molecules during AFM scanning. We demonstrate its usefulness by imaging cancerous B-cells as well as cell-junction molecules associated with living epithelial cells at high resolution. This technology can not only be used for imaging, but also for nanomanipulation. Current results suggest that this AFM nanomanipulation system and the novel cell fixture apparatus can be used to study the dynamic changes and function of cellular structures with unparalleled resolution both under physiological and pathophysiological conditions.

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Micro fixture enabled in-situ imaging and manipulation of cell membrane protein

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Keywords

ASJC Scopus subject areas

Access to Document

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